Induzierte gerichtete Vaskularisation mit einer arteriovenösen Gefäßschleife in einer biogenen Matrix zum Knochengewebsersatz durch Tissue Engineering
| Autor: | K. Heidner, A. D. Bach, R. E. Horch, J. Ohnolz, E. Polykandriotis, U. Kneser, J. Kopp |
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| Jazyk: | němčina |
| Rok vydání: | 2005 |
| Předmět: | |
| Zdroj: | 122. Kongress der Deutschen Gesellschaft für Chirurgie; 20050405-20050408; München; DOC05dgch3058 /20050615/ Chirurgisches Forum 2005 ISBN: 3540248889 |
| Popis: | Introduction: Vascularization still remains the main obstacle towards generation of larger volume bioartificial bone tissues. Axial vascularity may allow transfer of these neo tissues even under problematic clinical conditions. Aim of the current study was the induction of axial vascularization in a biogenic matrix. Methods: Discs of processed bovine cancellous bone (PBCB) were implanted into Lewis rats. In group A upon implantation, the matrix was inserted into a microsurgically constructed arteriovenous loop between the femoral artery and vein using a vein graft from the contralateral side. In group B the vascular carrier had the form of a distally ligated femoral arteriovenous bundle. In group C there was no vascular carrier. The matrices were encased into an isolation chamber made out of teflon. At 2, 4 and 8 weeks after implantation 4 matrices per group and time were explanted and subjected to histological and morphometric analysis. One matrix per group was subjected to corrosion casting. Injection of India ink into the abdominal aorta preceded explantations. Results were compared with dynamic MRI and vascular corrosion casts. Results: In group A nearly complete vascularization of the PBCB matrix occurred between the 4th and 8th week after implantation. At eight weeks, vascular remodelling with organization into vessels of various orders was evident. There was a significant superiority of group A in comparison to groups B and C in terms of vascular density and vascular meshwork morphology. Discussion: This study demonstrates for the first time vascularization of PBCB solid matrices by means of an AV loop. Injection of osteogenic cells into axially prevascularized matrices may eventually create functional bioartificial bone tissues for clinical application in large defects. |
| Databáze: | OpenAIRE |
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