Identification, sequence and mRNA expression pattern during metamorphosis of a cDNA encoding a glycine-rich cuticular protein in Tenebrio molitor
| Autor: | Hervé Bouhin, Annie Courrent, Jean Delachambre, Brigitte Quennedey, Jocelyne Mathelin |
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| Rok vydání: | 1995 |
| Předmět: |
Cuticle
Molecular Sequence Data Gene Expression Biology Complementary DNA Gene expression Genetics Protein biosynthesis Animals Tissue Distribution Amino Acid Sequence RNA Messenger Tenebrio Peptide sequence In Situ Hybridization chemistry.chemical_classification Expression vector Base Sequence Metamorphosis Biological Proteins Sequence Analysis DNA General Medicine Molecular biology Amino acid chemistry Protein Biosynthesis Ecdysis Insect Proteins |
| Zdroj: | Gene. 156:259-264 |
| ISSN: | 0378-1119 |
| DOI: | 10.1016/0378-1119(94)00005-d |
| Popis: | The study of insect cuticular proteins and their sequences is of interest because they are involved in protein-protein and protein-chitin interactions which confer the mechanical properties and fine architecture of the cuticle. Moreover, in the coleopteran Tenebrio molitor there is a dramatic change in cuticular architecture between pre- and postecdysial secretion. We report the isolation, by differential screening, and the sequence characterization of a cDNA clone encoding a cuticular protein of T. molitor, ACP17. After insertion in the expression vector pEX1, the recognition of the fusion protein by an anti-cuticular monoclonal antibody confirmed the cuticular nature of ACP17. Northern hybridization analysis showed that ACP17 mRNA expression begins weakly 3 days before adult ecdysis and strongly increases during the secretion of postecdysial adult cuticle, with a maximum just after ecdysis. In situ hybridization revealed that the ACP17 mRNA is only present in the epidermis which secretes hard cuticle. The deduced amino acid (aa) composition exhibits a high content of Gly (28%) and Ala (20%) and, particularly, two poly(Gx) stretches separated by repetitive motifs with proline AAPVA. A comparison is made with other cuticle aa sequences. |
| Databáze: | OpenAIRE |
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