Rat Erythrocytes Express the Anionic Amino Acid Transport Protein EAAC1
| Autor: | Mark Beveridge, Jill Verlander-Reed, Donald A. Novak |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
chemistry.chemical_classification
Erythrocytes Symporters Amino Acid Transport System X-AG Immunoelectron microscopy Peptide Cell Biology Hematology Biology Immunohistochemistry Rats Amino acid Rats Sprague-Dawley Glutamate Plasma Membrane Transport Proteins Amino Acid Transport Protein Excitatory Amino Acid Transporter 3 Membrane chemistry Biochemistry Animals Molecular Medicine Molecular Biology |
| Zdroj: | Blood Cells, Molecules, and Diseases. 29:261-266 |
| ISSN: | 1079-9796 |
| Popis: | ABSTRACT EAAC1 is a widely expressed protein which transports anionic amino acids in a Na + -dependent fashion. Rat erythrocytes have generally been thought to be impermeant to anionic amino acids. Utilizing immunoelectron microscopy, we have demonstrated the presence of EAAC1-immunoreactive protein within rat erythrocytes. Immunoblotting revealed the presence of an approximately 60-kDa protein, consistent with EAAC1, in erythrocyte membranes. Specificity was confirmed by peptide competition. In conclusion, EAAC1 is expressed in rat erythrocytes. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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