The major allergen of sesame seeds (Sesamum indicum) is a 2S albumin
| Autor: | Donatella Fortunato, Elena Varin, Claudio Ortolani, Federica Rivolta, Anna M. Robino, Chiara Trambaioli, Ambra Marianna Calamari, Maria Gabriella Giuffrida, Valerio Pravettoni, Luisa Lacava, Amedeo Conti, Laura Farioli, Elide A. Pastorello |
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| Rok vydání: | 2001 |
| Předmět: |
Adult
Male Allergy Blotting Western medicine.disease_cause Immunoglobulin E Magnoliopsida Allergen Albumins medicine Humans Sesamum Isoelectric Point Child Polyacrylamide gel electrophoresis Chromatography High Pressure Liquid Skin Tests Gel electrophoresis Chromatography biology Chemistry Albumin General Chemistry Allergens Antigens Plant medicine.disease biology.organism_classification Molecular Weight Biochemistry Seeds Chromatography Gel biology.protein Electrophoresis Polyacrylamide Gel Female Anaphylaxis 2S Albumins Plant |
| Zdroj: | Journal of Chromatography B: Biomedical Sciences and Applications. 756:85-93 |
| ISSN: | 0378-4347 |
| DOI: | 10.1016/s0378-4347(01)00073-1 |
| Popis: | Background: Allergic reactions induced by ingestion of foods containing sesame seeds are a well recognized cause of severe food-induced anaphylaxis. Objective: This study aimed to identify and characterize the clinically most important major allergen of sesame seeds. Methods: Sodium dodecyl sulfate–polyacrylamide gel electrophoresis and IgE immunoblotting were performed on sera of 10 patients selected for severe and documented allergic reaction after eating food containing sesame. The major allergen was purified by gel filtration and characterized by isoelectric point (pI), glycosylation and amino acid sequencing. Results: All the patients had positive IgE antibodies and skin prick tests (SPTs) to sesame. The major, clinically most important allergen was a protein with molecular mass of about 9000. It was not glycosylated, the amino acid sequence showed it was a 2S albumin with a pI of 7.3; the small and the large subunits, forming the whole protein, showed pI values of 6.5 and 6.0. |
| Databáze: | OpenAIRE |
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