Early commitment of adoptively transferred CD4+ T cells following particle-mediated DNA vaccination: implications for the study of immunomodulation
| Autor: | Benjamin M. Chain, Cathy A. van Wely, Tite John Philip, Remi J. Creusot, Lindy L. Thomsen, Peter Topley |
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| Rok vydání: | 2001 |
| Předmět: |
CD4-Positive T-Lymphocytes
Adoptive cell transfer Ovalbumin T cell Mice Transgenic Biology Lymphocyte Activation DNA vaccination Interferon-gamma Mice Interleukin 21 Th2 Cells Antigen Vaccines DNA medicine Animals Cytotoxic T cell Mice Inbred BALB C General Veterinary General Immunology and Microbiology ELISPOT Vaccination Public Health Environmental and Occupational Health Receptors Interleukin-2 T lymphocyte Th1 Cells Adoptive Transfer Molecular biology Microspheres Clone Cells Kinetics Infectious Diseases medicine.anatomical_structure Immunology Interleukin-2 Molecular Medicine Interleukin-4 Lymph Nodes Interleukin-5 Biomarkers Cell Division |
| Zdroj: | Vaccine. 19:1678-1687 |
| ISSN: | 0264-410X |
| Popis: | The early responses of CD4+ T cells to particle-mediated DNA immunisation were investigated using OVA-specific TCR-transgenic CD4+ T cells. Following adoptive transfer of these cells, mice were immunised by delivery into the skin of a plasmid encoding ovalbumin. Transgenic T cells underwent a rapid and transient antigen-specific activation, followed by clonal expansion (up to approximately 6% of total lymphocytes). Immunisation with ovalbumin in CFA evoked similar responses with slightly faster kinetics. Numerous antigen-specific T cells synthesising IFN-gamma (Th1) and IL-4 (Th2) were detectable using both intracellular staining and ELISPOT assays. This study provides a quantitative analysis of both T cell proliferation and Th1/Th2 balance following particle-mediated DNA immunisation and establishes a robust and sensitive model in which to assess modulation of helper T cell responses in DNA vaccination. |
| Databáze: | OpenAIRE |
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