Mode of Delivery Shapes Gut Colonization Pattern and Modulates Regulatory Immunity in Mice
| Autor: | Jane Preuss Hasselby, Camilla Hartmann Friis Hansen, Łukasz Krych, Lars Hestbjerg Hansen, Dennis Sandris Nielsen, Stine Broeng Metzdorff, Line S. F. Andersen, Karsten Buschard, Axel Kornerup Hansen, Søren Skov |
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| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
Male
Immunology Gut flora Adaptive Immunity T-Lymphocytes Regulatory Microbiology Diabetes Mellitus Experimental Mice Immune system Mice Inbred NOD Ruminococcus medicine Immunology and Allergy Animals Bacteroides NOD mice Clostridium Mice Inbred BALB C Mucous Membrane biology Cesarean Section Microbiota Lachnospiraceae FOXP3 biology.organism_classification Acquired immune system Delivery mode medicine.disease Intestines Mice Inbred C57BL Female Insulitis |
| Zdroj: | H. F. Hansen, C, S. F. Andersen, L, Krych, Ł, B. Metzdorff, S, P. Hasselby, J, Skov, S, S. Nielsen, D, Buschard, K, Hansen, L H & Hansen, A J K 2014, ' Mode of Delivery Shapes Gut Colonization Pattern and Modulates Regulatory Immunity in Mice ', The Journal of Immunology, vol. 193, no. 3, pp. 1213-1222 . https://doi.org/10.4049/jimmunol.1400085 |
| DOI: | 10.4049/jimmunol.1400085 |
| Popis: | Delivery mode has been associated with long-term changes in gut microbiota composition and more recently also with changes in the immune system. This has further been suggested to link Cesarean section (C-section) with an increased risk for development of immune-mediated diseases such as type 1 diabetes. In this study, we demonstrate that both C-section and cross-fostering with a genetically distinct strain influence the gut microbiota composition and immune key markers in mice. Gut microbiota profiling by denaturing gradient gel electrophoresis and 454/FLX-based 16S rRNA gene amplicon sequencing revealed that mice born by C-section had a distinct bacterial profile at weaning characterized by higher abundance of Bacteroides and Lachnospiraceae, and less Rikenellaceae and Ruminococcus. No clustering according to delivery method as determined by principal component analysis of denaturing gradient gel electrophoresis profiles was evident in adult mice. However, the adult C-section–born mice had lower proportions of Foxp3+ regulatory T cells, tolerogenic CD103+ dendritic cells, and less Il10 gene expression in mesenteric lymph nodes and spleens. This demonstrates long-term systemic effect on the regulatory immune system that was also evident in NOD mice, a model of type 1 diabetes, born by C-section. However, no effect of delivery mode was seen on diabetes incidence or insulitis development. In conclusion, the first exposure to microorganisms seems to be crucial for the early life gut microbiota and priming of regulatory immune system in mice, and mode of delivery strongly influences this. |
| Databáze: | OpenAIRE |
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