Use of an ELISA for differential diagnosis of Mycoplasma agalactiae and M mycoides subspecies mycoides (LC) in naturally infected goat herds
| Autor: | S. Levisohn, I. Davidson, E. Rapoport, M.-R. Caro Vergara |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
Keratoconjunctivitis
Infectious Mycoplasma agalactiae ved/biology.organism_classification_rank.species Immunoblotting Mycoplasmataceae Enzyme-Linked Immunosorbent Assay Mastitis medicine.disease_cause Microbiology Serology Diagnosis Differential Epitopes Mycoplasma medicine Animals Mycoplasma Infections Seroconversion Antigens Bacterial Arthritis Infectious Goat Diseases General Veterinary biology ved/biology Goats Mycoplasma mycoides Syndrome biology.organism_classification medicine.disease Virology Antibodies Bacterial Milk Carrier State Mollicutes Electrophoresis Polyacrylamide Gel |
| Zdroj: | Research in veterinary science. 51(1) |
| ISSN: | 0034-5288 |
| Popis: | Contagious agalactia is an ovine and caprine mycoplasmosis which manifests as mastitis, arthritis and keratoconjunctivitis. Mycoplasma agalactiae is recognised as a causal agent but M mycoides sub-species mycoides ( LC ), and M capricolum may also be responsible for this syndrome in goats. The clinical signs are not pathognomonic; diagnostic procedures are based on isolation of the organism from diseased animals or by detection of seroconversion. An ELISA specific for M agalactiae and M m mycoides ( LC ) is described. The specificity of the antigens was demonstrated by immunoblotting and by ELISA using mono-specific hyperimmune rabbit sera. A correlation of ELISA activity with other serological tests and isolation of mycoplasmas was carried out in two goat herds under field conditions. Results indicate the ability to detect subclinical mycoplasma infection and individual carrier goats on the basis of ELISA , a finding which will assist control procedures. |
| Databáze: | OpenAIRE |
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