Gastroprotective effect of ethanol extracts of cladodes and roots of Pilosocereus gounellei (A. Weber ex K. Schum.) Bly. Ex Rowl (Cactaceae) on experimental ulcer models
| Autor: | Antônio Carlos Melo Lima Filho, Tânia Silva, Maurício P.M. Amaral, Rita de Cássia Meneses Oliveira, Francisco Valmor Macedo Cunha, Francisco Rodrigo de Asevedo Mendes de Oliveira, Glaubert A. Sousa, M. F. Souza, Irisdalva S. Oliveira, Jéssica Karina da Silva Maciel, Pedro Dantas Fernandes, Benedito P.S. Neto, Francilene V. Silva-Freitas, Ana Flávia Seraine Custódio Viana, Rodrigo Lopes Gomes Gonçalves |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Cactaceae Male Restraint Physical Nitric Oxide 030226 pharmacology & pharmacy Plant Roots Nitric oxide 03 medical and health sciences chemistry.chemical_compound Mice 0302 clinical medicine Drug Discovery medicine Cladodes Animals Stomach Ulcer Rats Wistar Pharmacology Ethanol biology Traditional medicine Plant Extracts Stomach biology.organism_classification Pylorus Anti-Ulcer Agents Catalase Acute toxicity Cold Temperature Disease Models Animal 030104 developmental biology NPSH medicine.anatomical_structure chemistry Gastric Mucosa Reperfusion Injury biology.protein Prostaglandins Solvents Gastritis medicine.symptom Phytotherapy |
| Zdroj: | Journal of ethnopharmacology. 218 |
| ISSN: | 1872-7573 |
| Popis: | Ethnopharmacological relevance Pilosocereus gounellei Cactaceae), popularly known as “xique xique”, is a species native from Caatinga region of Northeast Brazil, which is used by traditional communities in folk medicine for a variety of health problems, especially inflammatory processes and gastritis. Aim of the study The present study investigates the possible gastric antiulceractivity of ethanol extracts obtained from the cladodes and roots of Pilosocereus gounellei (EECPG and EERPG, respectively) and mechanisms of action underlying this effect. Materials and methods Mice were used for the evaluation of the acute toxicity, and mice and rats to study the gastroprotective activity. The gastroprotective action of EECPG and EERPG was analyzed in the absolute ethanol in mice, ischemia-reperfusion and cold restraint stress in rats. In the investigation of the gastroprotective mechanisms of EECPG and EERPG, the participation of the NO and prostaglandins, the levels of the non-protein sulfhydril groups (NP-SH) and the catalase activity using the ethanol-induced gastric mucosa lesion model and the quantification of the gastric mucus and the antisecretory activity through pylorus ligature model in rats were analyzed. Results The animals did not present any signs of acute toxicity for the EECPG and EERPG, and it was not possible to calculate the DL50. EECPG and EERPG (200 and 400 mg/kg) exhibited a significant gastroprotective effect in absolute ethanol, ischemia-reperfusion-induced and cold restraint stress gastric lesion models. Gastroprotection of EECPG and EERPG (200 mg/kg) was significantly decreased in pre-treated mice with L-NAME. Our studies revealed that EECPG and EERPG (200 mg/kg) prevented the decrease of the non-protein sulfhydril groups (NPSH) and increased the catalase levels in ethanol-treated animals. However, the gastric secretion parameters (volume, [H+], pH) did not show any alteration. Conclusions Our results indicate that the ethanolic extract from the cladodes and roots of Pilosocereus gounellei exhibits a significant gastroprotection, because it inhibits the formation of gastric lesions using different models. The participation of the nitric oxide, prostaglandins, the non-protein sulfhydril groups (NP-SH), catalase seem to be involved in the gastroprotection activity of the EECPG and EERPG. Nevertheless, this activity does not seem to be related to antisecretory mechanisms. |
| Databáze: | OpenAIRE |
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