Wireless transmission of fast-scan cyclic voltammetry at a carbon-fiber microelectrode: proof of principle
Autor: | Paul E. Langley, Phillip G. Greco, Stefan G. Sandberg, George V. Rebec, Paul A. Garris, Andy Alexander, John Poehlman, Robert Ensman, R. Mark Wightman |
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Rok vydání: | 2003 |
Předmět: |
Male
Time Factors Computer science Dopamine Fast-scan cyclic voltammetry Analytical chemistry law.invention Membrane Potentials Bluetooth Rats Sprague-Dawley law Carbon Fiber Software Design Electronic engineering Wireless Animals Telemetry business.industry General Neuroscience Brain Extracellular Fluid Neurochemistry Signal Processing Computer-Assisted Carbon Rats Electrophysiology Microelectrode Capacitor Proof of concept Biological Assay Resistor Electronics business Artifacts Microelectrodes Data transmission |
Zdroj: | Journal of neuroscience methods. 140(1-2) |
ISSN: | 0165-0270 |
Popis: | Fast-scan cyclic voltammetry (FSCV) at a carbon-fiber microelectrode (CFM) provides exquisite temporal and spatial resolution for monitoring brain chemistry. The utility of this approach has recently been demonstrated by measuring sub-second dopamine changes associated with behavior. However, one drawback is the cable link between animal and recording equipment that restricts behavior and precludes monitoring in complex environments. As a first step towards developing new instrumentation to overcome this technical limitation, the goal of the present study was to establish proof of principle for the wireless transmission of FSCV at a CFM. Proof of principle was evaluated in terms of measurement stability, fidelity, and susceptibility to ambient electrical noise. Bluetooth digital telemetry provided bi-directional communication between remote and home-base units and stable, high-fidelity data transfer comparable to conventional, wired systems when tested using a dummy cell (i.e., a resistor and capacitor in series simulating electrical properties of a CFM), and dopamine measurements with flow injection analysis and in the anesthetized rat with electrical stimulation. The wireless system was also less susceptible to interference from ambient electrical noise. Taken together, the present findings establish proof of principle for the wireless transmission of FSCV at a CFM. |
Databáze: | OpenAIRE |
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