Regional Expression of Epithelial Dipeptidyl Peptidase IV in the Human Intestines
| Autor: | Yangxi Wang, A. Couvineau, T. Voisin, C Rouyer-Fessard, Dallas M. Swallow, D. Darmoul, M. Laburthe, R. Salomon |
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| Rok vydání: | 1994 |
| Předmět: |
Adult
Colon Duodenum Dipeptidyl Peptidase 4 Blotting Western Molecular Sequence Data Biophysics Gene Expression Ileum Biology Polymerase Chain Reaction digestive system Biochemistry Epithelium Dipeptidyl peptidase Jejunum Gene expression medicine Humans RNA Messenger Northern blot Dipeptidyl-Peptidases and Tripeptidyl-Peptidases Molecular Biology Base Sequence digestive oral and skin physiology RNA-Directed DNA Polymerase Cell Biology Blotting Northern Molecular biology Small intestine Intestines Blot medicine.anatomical_structure |
| Zdroj: | Biochemical and Biophysical Research Communications. 203:1224-1229 |
| ISSN: | 0006-291X |
| DOI: | 10.1006/bbrc.1994.2313 |
| Popis: | The expression of DPP IV/CD26 was studied in epithelial cells from different regions of the human small intestine and colon. The following data were obtained: 1) DPP IV/CD26 activity was highest in ileum and jejunum, low in duodenum and not detectable in colon; 2) The amount of DPP IV, as analyzed by Western blotting, was very low in duodenum and increased up to the ileum. No DPP IV protein was detected in colon; 3) RT-PCR analysis showed the expected 315-bp product in the small intestine with the highest signal in ileum and jejunum and a low signal in duodenum; a faint PCR signal could be detected in colon. Similar data were obtained by Northern blot analysis which revealed a 3.6-kb transcript only in small intestine. It is concluded that DPP IV/CD26 gene expression in human intestine is highest in the distal small intestine, and that is regulated at the mRNA level. |
| Databáze: | OpenAIRE |
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