Overexpression of NgAUREO1, the gene coding for aurechrome 1 from Nannochloropsis gaditana, into Saccharomyces cerevisiae leads to a 1.6-fold increase in lipid accumulation
| Autor: | Ming-gang Zheng, Li Zheng, Ling Wang, Yi-jiang Huang, Yan-li Tong, Yan Li |
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| Rok vydání: | 2013 |
| Předmět: |
Saccharomyces cerevisiae
Gene Expression Bioengineering Applied Microbiology and Biotechnology law.invention chemistry.chemical_compound law Coenzyme A Ligases Diacylglycerol O-Acyltransferase Gene Transcription factor Diacylglycerol kinase Fatty acid metabolism biology Gene Expression Profiling Acetyl-CoA carboxylase General Medicine Lipid Metabolism biology.organism_classification Lipids Recombinant Proteins Pyruvate carboxylase chemistry Biochemistry Recombinant DNA lipids (amino acids peptides and proteins) Stramenopiles Acetyl-CoA Carboxylase Transcription Factors Biotechnology |
| Zdroj: | Biotechnology Letters. 36:575-579 |
| ISSN: | 1573-6776 0141-5492 |
| DOI: | 10.1007/s10529-013-1386-0 |
| Popis: | Aureochrome-1 (AUREO1) is a transcription factor that is induced by blue light and controls branching of Vaucheria frigida. We have cloned the gene, NgAUREO1, coding for AUREO1 from Nannochloropsis gaditana, and report that the lipid content in recombinant Saccharomyces cerevisiae was 1.6-fold more than in wild-type S. cerevisiae (6.3 % lipid increased to 10 %). Over-expression of AUREO1 in S. cerevisiae up-regulated the expression of acetyl-CoA carboxylase and acyl-CoA:diacylglycerol acyl-transferase but down-regulated the expression of long-chain-acyl CoA synthetase. This enhanced the accumulation of lipid. This study highlights a novel function of AUREO1 and allows a better understanding of the regulation mechanism of fatty acid metabolism. |
| Databáze: | OpenAIRE |
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