Prolactin selectively transported to cerebrospinal fluid from blood under hypoxic/ischemic conditions

Autor: Tomoya Ikeda, Akihiro Ohyama, Miho Watanabe, Takaki Ishikawa, Junko Toyomura, Naoto Tani
Rok vydání: 2018
Předmět:
Male
Vascular Endothelial Growth Factor A
0301 basic medicine
Pituitary gland
Critical Care and Emergency Medicine
Physiology
lcsh:Medicine
Miniature swine
Pathology and Laboratory Medicine
Nervous System
chemistry.chemical_compound
0302 clinical medicine
Cerebrospinal fluid
Ischemia
Medicine and Health Sciences
Child
Hypoxia
lcsh:Science
Trauma Medicine
Cerebrospinal Fluid
Aged
80 and over
Multidisciplinary
Brain
Middle Aged
Body Fluids
Vascular endothelial growth factor
Head Injury
Protein Transport
Blood
medicine.anatomical_structure
Pituitary Gland
Child
Preschool
Female
Choroid plexus
Anatomy
medicine.symptom
Burns
Traumatic Injury
hormones
hormone substitutes
and hormone antagonists
Research Article
Adult
endocrine system
medicine.medical_specialty
Adolescent
Endocrine System
Cell Line
Asphyxia
Young Adult
03 medical and health sciences
Signs and Symptoms
Diagnostic Medicine
Internal medicine
medicine
Humans
RNA
Messenger
Secretion
Aged
business.industry
lcsh:R
Biology and Life Sciences
Infant
Hypoxia (medical)
medicine.disease
Survival Analysis
Prolactin
Neuroanatomy
030104 developmental biology
Endocrinology
Gene Expression Regulation
chemistry
Choroid Plexus
lcsh:Q
Physiological Processes
business
030217 neurology & neurosurgery
Neuroscience
Zdroj: PLoS ONE, Vol 13, Iss 6, p e0198673 (2018)
PLoS ONE
ISSN: 1932-6203
Popis: Aim The aim of this study was to determine and to verify the correlation between the amount of prolactin (PRL) levels in the blood and in the cerebrospinal fluid (CSF) by various causes of death as an indicator for acute hypoxia in autopsy cases. It is to confirm the cause of the change in prolactin level in CSF by in vitro system. Materials and methods In autopsy materials, the PRL levels in blood from the right heart ventricle and in the CSF were measured by chemiluminescent enzyme immunoassay, and changes in the percentage of PRL-positive cells in the pituitary gland were examined using an immunohistochemical method. Furthermore, an inverted culture method was used as an in vitro model of the blood-CSF barrier using epithelial cells of the human choroid plexus (HIBCPP cell line) and SDR-P-1D5 or MSH-P3 (PRL-secreting cell line derived from miniature swine hypophysis) under normoxic or hypoxic (5% oxygen) conditions, and as an index of cell activity, we used Vascular Endothelial Growth Factor (VEGF). Results and discussion Serum PRL levels were not significantly different between hypoxia/ischemia cases and other causes of death. However, PRL levels in CSF were three times higher in cases of hypoxia/ischemia than in those of the other causes of death. In the cultured cell under the hypoxia condition, PRL and VEGF showed a high concentration at 10 min. We established a brain-CSF barrier model to clarify the mechanism of PRL transport to CSF from blood, the PRL concentrations from blood to CSF increased under hypoxic conditions from 5 min. These results suggested that PRL moves in CSF through choroidal epithelium from blood within a short time. PRL is hypothesized to protect the hypoxic/ischemic brain, and this may be because of the increased transportation of the choroid plexus epithelial cells.
Databáze: OpenAIRE
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