Redescription of Monacha pantanellii (De Stefani, 1879), a species endemic to the central Apennines, Italy (Gastropoda, Eupulmonata, Hygromiidae) by an integrative molecular and morphological approach
| Autor: | Alessandro Hallgass, Debora Barbato, Folco Giusti, Andrzej Lesicki, Giuseppe Manganelli, Ewa Kosicka, Joanna R. Pieńkowska |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
0301 basic medicine Range (biology) shell and genital structure Gastropoda Species distribution ITS2 Orthurethra Helicoidea 010603 evolutionary biology 01 natural sciences Plectopyloidea COI 03 medical and health sciences 16S rDNA lcsh:Zoology Animalia Eupulmonata lcsh:QL1-991 Wiwaxia Hygrophila Clade Ecology Evolution Behavior and Systematics Hygromiidae biology Phylogenetic tree Monacha Cephalornis Isognomostoma isognomostomos Monacha cantiana biology.organism_classification Pulmonata H3 Stylommatophora 030104 developmental biology Mollusca Heterobranchia Evolutionary biology Animal Science and Zoology species distribution 16S rDNA COI H3 ITS2 molecular features shell and genital structure species distribution molecular features |
| Zdroj: | ZooKeys, Vol 988, Iss, Pp 17-61 (2020) ZooKeys 988: 17-61 |
| ISSN: | 1313-2970 |
| Popis: | Specimens obtained from ten populations of a Monacha species from the central Apennines were compared with six molecular lineages of Monacha cantiana s. l. (CAN-1, CAN-2, CAN-3, CAN-4, CAN-5, CAN-6) and two other Monacha species (M. cartusiana and M. parumcincta), treated as outgroup, by molecular (nucleotide sequences of two mitochondrial COI and 16S rDNA as well as two nuclear ITS2 and H3 gene fragments) and morphological (shell and genital anatomy) analysis. The results strongly suggest that these populations represent a separate species for which two names are available: the older Helix pantanellii De Stefani, 1879 and the junior M. ruffoi Giusti, 1973. The nucleotide sequences created well separated clades on each phylogenetic tree. Genital anatomy included several distinctive features concerning vaginal appendix, penis, penial papilla and flagellum; instead, shell characters only enabled them to be distinguished from M. cartusiana and M. parumcincta. Remarkably, populations of M. pantanellii show high morphological variability. Shell variability mainly concerns size, some populations having very small dimensions. Genital variability shows a more intricate pattern of all anatomical parts, being higher as regards the vagina and vaginal appendix. Despite this morphological variability, the K2P distance range of COI sequences between populations is narrow (0.2–4.5%), if we consider all but three of the 53 sequences obtained. This research confirmed that the species of Monacha and their molecularly distinguished lineages can only occasionally be recognised morphologically and that they have significant inter- and intra-population variability. The possibility of using an overall approach, including shell, genital and molecular evidence, was taken in order to establish a reliable taxonomic setting. |
| Databáze: | OpenAIRE |
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