Assessment of Nuclear Nanomorphology Marker to Improve the Detection of Malignancy From Bile Duct Biopsy Specimens
| Autor: | Rajan K. Bista, Douglas J. Hartman, Kevin D. Staton, Sumera Rizvi, Yang Liu, Adam Slivka, Shikhar Uttam, Alyssa M. Krasinskas, Randall E. Brand |
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| Rok vydání: | 2014 |
| Předmět: |
Adult
Male medicine.medical_specialty Pathology Biopsy Bile Duct Neoplasm Biology Malignancy Sensitivity and Specificity Article Young Adult Biomarkers Tumor medicine Humans In patient Early Detection of Cancer Aged Aged 80 and over Cell Nucleus medicine.diagnostic_test Bile duct Cancer Histology General Medicine Middle Aged Molecular diagnostics medicine.disease Nanostructures medicine.anatomical_structure Bile Duct Neoplasms Female Bile Ducts Radiology |
| Zdroj: | American Journal of Clinical Pathology. 141:884-891 |
| ISSN: | 1943-7722 0002-9173 |
| DOI: | 10.1309/ajcpxqd0nsjnk0cx |
| Popis: | Objectives: The accurate diagnosis of malignancy from small bile duct biopsy specimens is often challenging. This proof-of-concept study assessed the feasibility of a novel optical technology, spatial-domain low-coherence quantitative phase microscopy (SL-QPM), that assesses nanoscale structural alterations in epithelial nuclei for improving the diagnosis of malignancy in bile duct biopsy specimens. Methods: The SL-QPM analysis was performed on standard histology specimens of bile duct biopsy specimens from 45 patients. We analyzed normal cells with benign follow-up, histologically normal cells with pancreaticobiliary malignancy, and malignant epithelial cells. Results: The SL-QPM–derived nuclear nanomorphology marker can not only distinguish benign and malignant epithelial cells but can also detect features of malignancy in those cells normal by light microscopy with a discriminatory accuracy of 0.90. When combining pathology with SL-QPM, the sensitivity is improved to 88.5% from 65.4% of conventional pathology, while maintaining 100% specificity. Conclusions: SL-QPM–derived nuclear nanomorphology markers represent a novel approach for detecting malignancy from histologically normal-appearing epithelial cells, with potential as an adjunctive test in patients with negative or inconclusive pathologic diagnosis on bile duct biopsy specimens. |
| Databáze: | OpenAIRE |
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