Injectable thermosensitive hydrogel containing hyaluronic acid and chitosan as a barrier for prevention of postoperative peritoneal adhesion
| Autor: | Chien-Hung Liao, Chih-Hao Chen, Jyh-Ping Chen, Shih-Hsien Chen, Ming-Jin Tsai, Pang-Yun Chou, Shih-Hsuan Mao |
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| Rok vydání: | 2017 |
| Předmět: |
Male
Polymers and Plastics Tissue Adhesions 02 engineering and technology 010402 general chemistry 01 natural sciences Rats Sprague-Dawley Chitosan Mice chemistry.chemical_compound Postoperative Complications Hyaluronic acid Materials Chemistry Animals Hyaluronic Acid chemistry.chemical_classification Tissue Adhesion Organic Chemistry Temperature Hydrogels Polymer Penetration (firestop) 021001 nanoscience & nanotechnology Acute toxicity Rats 0104 chemical sciences chemistry Cell culture NIH 3T3 Cells Peritoneal adhesion Peritoneum 0210 nano-technology Biomedical engineering |
| Zdroj: | Carbohydrate Polymers. 173:721-731 |
| ISSN: | 0144-8617 |
| DOI: | 10.1016/j.carbpol.2017.06.019 |
| Popis: | Peritoneal adhesion is one of the common complications after abdominal surgery. Injectable thermosensitive hydrogel could serve as an ideal barrier to prevent this postoperative tissue adhesion. In this study, poly(N-isopropylacrylamide) (PNIPAm) was grafted to chitosan (CS) and the polymer was further conjugated with hyaluronic acid (HA) to form thermosensitive HA-CS-PNIPAm hydrogel. Aqueous solutions of PNIPAm and HA-CS-PNIPAm at 10%(w/v) are both free-flowing and injectable at room temperature and exhibit sol-gel phase transition around 31°C; however, HA-CS-PNIPAm shows less volume shrinkage after gelation and higher complex modulus than PNIPAm. Cell culture studies indicate both injectable hydrogel show barrier effects to reduce fibroblasts penetration while induce little cytotoxicity in vitro. From a sidewall defect-bowel abrasion model in rats, significant reduction of postoperative peritoneal adhesion was found for peritoneal defects treated with HA-CS-PNIPAm compared with those treated with PNIPAm and untreated controls from gross and histological evaluation. Furthermore, HA-CS-PNIPAm did not interfere with normal peritoneal tissue healing and did not elicit acute toxicity from blood analysis and tissue biopsy examination. By taking advantage of the easy handling and placement properties of HA-CS-PNIPAm during application, this copolymer hydrogel would be a potentially ideal injectable anti-adhesion barrier after abdominal surgeries. |
| Databáze: | OpenAIRE |
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