Changes in endotoxin-induced cytokine production by whole blood after in vivo exposure of normal humans to endotoxin
| Autor: | S. M. Coyle, T van der Poll, S. F. Lowry, L. L. Moldawer |
|---|---|
| Přispěvatelé: | Other departments |
| Jazyk: | angličtina |
| Rok vydání: | 1996 |
| Předmět: |
Adult
Lipopolysaccharides Male medicine.medical_specialty Lipopolysaccharide medicine.drug_class chemistry.chemical_compound In vivo Internal medicine Immune Tolerance Humans Immunology and Allergy Medicine Whole blood Dose-Response Relationship Drug Interleukin-6 Tumor Necrosis Factor-alpha business.industry Vaccination Receptors Interleukin-1 Interleukin Receptor antagonist Interleukin-10 Interleukin 10 Blood Infectious Diseases Endocrinology chemistry Immunology Tumor necrosis factor alpha lipids (amino acids peptides and proteins) business Ex vivo Interleukin-1 |
| Zdroj: | Journal of infectious diseases, 174(6), 1356-1360. Oxford University Press |
| ISSN: | 0022-1899 |
| DOI: | 10.1093/infdis/174.6.1356 |
| Popis: | Whole blood of 6 healthy subjects, who were intravenously injected with lipopolysaccharide (LPS, 2 ng/kg), was stimulated ex vivo with LPS (10 ng/mL). Three and 6 h after injection of LPS, whole blood produced less tumor necrosis factor-alpha (TNF), interleukin (IL)-1beta, IL-6, and IL-10 (all P < .05). By contrast, the production of IL-1 receptor antagonist was enhanced after LPS injection (P < .05). Plasma obtained 2 h, but not 1 h, after in vivo administration of LPS showed a dose-dependent inhibition of TNF, IL-1beta, and IL-6 production by LPS-stimulated whole blood from 6 other healthy donors not previously exposed to LPS, while the production of IL-10 and IL-1 receptor antagonist were not or were marginally influenced. LPS tolerance represents a purposeful adaptation of the host, rather than a generalized hyporesponsiveness, and is at least partly mediated by soluble factors produced within 2 h after previous exposure to LPS. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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