A flow-injection chemiluminescence method for the determination of human serum albumin, using the reaction of fluorescein–human serum albumin–sodium hypochlorite by the enhancement effect of the cationic surfactant cetyltrimethylammonium bromide
| Autor: | Sheng-Fu Wang, Chun-Bao Huang, Xue-Lian Liu, Kai Zhang |
|---|---|
| Rok vydání: | 2007 |
| Předmět: |
Sodium Hypochlorite
Biophysics Serum albumin Sensitivity and Specificity law.invention Surface-Active Agents chemistry.chemical_compound Pulmonary surfactant Bromide law Cations medicine Humans Fluorescein Serum Albumin Chemiluminescence Flow injection analysis Detection limit Chromatography Molecular Structure biology Cetrimonium Chemistry Hydrogen-Ion Concentration Human serum albumin body regions Kinetics Chemistry (miscellaneous) Flow Injection Analysis Luminescent Measurements embryonic structures Cetrimonium Compounds biology.protein medicine.drug |
| Zdroj: | Luminescence. 22:393-400 |
| ISSN: | 1522-7243 1522-7235 |
| DOI: | 10.1002/bio.975 |
| Popis: | The interaction between surfactant and fluorescein was studied, using a fluorescence spectroscopy and flow-injection (FI) chemiluminescence (CL) method. It was found that the cationic surfactant cetyltrimethylammonium bromide (CTAB) could cause the structural transformation of fluorescein from the quinone to the spirolactone form, and greatly enhance the CL intensity of the fluorescein-human serum albumin (HSA) complex. Based on this finding, a rapid and sensitive FI-CL method was developed for the determination of HSA. Under the optimum conditions, the proposed method has a linear range of 0.05-24.0 microg/mL, with a detection limit of 0.03 microg/mL for HSA (3sigma). The relative standard deviation (RSD) of 1.2 microg/mL HSA (n = 8) is 0.8%. The method was applied to the determination of protein content in urine samples, with satisfactory results. Density functional theory was used to study the mechanism of surfactant-enhanced CL intensity of the fluorescein-HSA complex. |
| Databáze: | OpenAIRE |
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