Target-enriched DNA sequencing from historical type material enables a partial revision of the Madagascar giant stream frogs (genus Mantidactylus)

Autor: Carl R. Hutter, Frank Glaw, Teddy Bruy, Franco Andreone, Nicolas Straube, Michaela Preick, Mark D. Scherz, Elvis Almeida Pereira, Mariana L. Lyra, Andolalao Rakotoarison, J. Christian Randrianantoandro, Angelica Crottini, Loïs Rancilhac, Jeffrey W. Streicher, Miguel Vences, Annemarie Ohler, Michael Hofreiter
Přispěvatelé: Institut de Systématique, Evolution, Biodiversité (ISYEB ), Muséum national d'Histoire naturelle (MNHN)-École pratique des hautes études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Université des Antilles (UA), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS), Museo Regionale di Scienze Naturali di Torino, Sezione di Zoologia, Zoologische Staatssammlung Muenchen (SNSB), Department of Evolutianory Genetics, Max-Planck-Institut, Technische Universität Braunschweig = Technical University of Braunschweig [Braunschweig], Braunschweig University of Technology, Zoologische Staatssammlung München (ZSM-SNSB), Sorbonne Université, Universidade Federal Rural do Rio de Janeiro, University of Potsdam, University Museum of Bergen, Universidade Estadual Paulista (Unesp), The Natural History Museum, Museo Regionale di Scienze Naturali, Universidade do Porto, University of Kansas, Université d’Antananarivo
Rok vydání: 2020
Předmět:
Zdroj: Journal of Natural History
Journal of Natural History, Taylor & Francis, 2020, pp.1-32. ⟨10.1080/00222933.2020.1748243⟩
Scopus
Repositório Institucional da UNESP
Universidade Estadual Paulista (UNESP)
instacron:UNESP
ISSN: 0022-2933
1464-5262
Popis: Made available in DSpace on 2020-12-12T01:22:39Z (GMT). No. of bitstreams: 0 Previous issue date: 2020-01-17 The subgenus Mantidactylus is a group of frogs endemic to Madagascar, including the largest anuran species on the island. Although these frogs are common and widely distributed, their taxonomy remains unclear. Two species are currently recognised, M. grandidieri and M. guttulatus, with another available name, Rana pigra, considered to be a synonym of M. grandidieri. However, molecular studies have suggested the presence of several cryptic species within the group. Additionally, due to the lack of prominent morphological features, allocating the available names to evolutionary lineages has proven challenging. In the present study, we take a first step towards solving these problems by using fragments of the 16S mitochondrial gene and RAG1 nuclear gene from all over the range of the subgenus to describe its genetic diversity. We also use a newly designed target enrichment laboratory protocol to sequence three mitochondrial fragments from five name-holding museum specimens (as old as 120 years) in order to determine to which lineages the existing names should be applied. The study of the 16S mitochondrial gene revealed 7 geographically separated lineages, distinct enough to be considered candidate species. Out of the five museum specimens analysed, four successfully yielded DNA sequences and could be attributed to one of the aforementioned lineages. Therefore, the name Mantidactylus grandidieri should be applied to the populations from North-Eastern Madagascar, while M. guttulatus refers to populations from inland localities along the Eastern coast of the island. On the other hand, the holotype of Rana pigra did not yield enough genetic material to allow definitive identification. While our data were not sufficient to assess the status of the four lineages distributed along the Eastern coast, the populations from North-Western Madagascar were highly distinct on both the mitochondrial and nuclear markers. We thus describe them as a new species, M. radaka sp. nov. http://www.zoobank.org/urn:lsid:zoobank.org:pub:1EDDAF0D-FE37-490A-B09E-E136A0C5CB35. Zoological Institute Braunschweig University of Technology Sektion Herpetologie Zoologische Staatssammlung München (ZSM-SNSB) Institut de Systématique Biodiversité (ISYEB) Sorbonne Université Programa de Pós-Graduação em Biologia Animal Departamento de Biologia Animal Laboratório de Herpetologia Universidade Federal Rural do Rio de Janeiro Faculty of Mathematics and Natural Sciences Institute for Biochemistry and Biology University of Potsdam Department of Natural History University Museum of Bergen Depto de Zoologia Instituto de Biologia Universidade Estadual Paulista - UNESP Department of Life Sciences The Natural History Museum, Cromwell Road Museo Regionale di Scienze Naturali Research Centre in Biodiversity Genetics and Evolution Universidade do Porto Biodiversity Institute and Department of Ecology and Evolutionary Biology University of Kansas Mention Zoologie et Biodiversité Animale Faculté des Sciences Université d’Antananarivo Depto de Zoologia Instituto de Biologia Universidade Estadual Paulista - UNESP
Databáze: OpenAIRE
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