A single-cell survey of the small intestinal epithelium

Autor: Grace Burgin, Adam L. Haber, Christopher Smillie, Danielle Dionne, Michael R. Howitt, Rebecca H. Herbst, Ömer H. Yilmaz, Toni Delorey, Orit Rozenblatt-Rosen, Hai Ning Shi, Noga Rogel, Mei Zhang, Aviv Regev, Wendy S. Garrett, Raktima Raychowdhury, Yarden Katz, Karthik Shekhar, Ramnik J. Xavier, Semir Beyaz, Itay Tirosh, Moshe Biton
Rok vydání: 2017
Předmět:
Male
0301 basic medicine
Paneth Cells
Cell type
Transcription
Genetic
General Science & Technology
Cellular differentiation
Cell
Enteroendocrine cell
Small
Epithelium
Vaccine Related
Mice
03 medical and health sciences
Immune system
Genetic
Thymic Stromal Lymphopoietin
Single-cell analysis
Intestine
Small
medicine
2.1 Biological and endogenous factors
Animals
Homeostasis
Aetiology
Multidisciplinary
biology
Gene Expression Profiling
Cell Differentiation
Epithelial Cells
biology.organism_classification
Intestine
3. Good health
Cell biology
Organoids
Emerging Infectious Diseases
Infectious Diseases
Enterocytes
030104 developmental biology
medicine.anatomical_structure
Immunology
Cytokines
Leukocyte Common Antigens
Female
Heligmosomoides polygyrus
Single-Cell Analysis
Tuft cell
Digestive Diseases
Transcription
Zdroj: Nature, vol 551, iss 7680
ISSN: 1476-4687
0028-0836
Popis: Intestinal epithelial cells absorb nutrients, respond to microbes, function as a barrier and help to coordinate immune responses. Here we report profiling of 53,193 individual epithelial cells from the small intestine and organoids of mice, which enabled the identification and characterization of previously unknown subtypes of intestinal epithelial cell and their gene signatures. We found unexpected diversity in hormone-secreting enteroendocrine cells and constructed the taxonomy of newly identified subtypes, and distinguished between two subtypes of tuft cell, one of which expresses the epithelial cytokine Tslp and the pan-immune marker CD45, which was not previously associated with non-haematopoietic cells. We also characterized the ways in which cell-intrinsic states and the proportions of different cell types respond to bacterial and helminth infections: Salmonella infection caused an increase in the abundance of Paneth cells and enterocytes, and broad activation of an antimicrobial program; Heligmosomoides polygyrus caused an increase in the abundance of goblet and tuft cells. Our survey highlights previously unidentified markers and programs, associates sensory molecules with cell types, and uncovers principles of gut homeostasis and response to pathogens.
Databáze: OpenAIRE
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