A B Cell-Based Sensor for Rapid Identification of Pathogens

Autor:	David J. Blanchard, Albert M. Young, Jianzhu Chen, Eric Schwoebel, Laura T. Bortolin, Martha S. Petrovick, Frances Nargi, Richard H. Mathews, James Harper, Mark A. Hollis, Todd H. Rider
Rok vydání:	2003
Předmět:	Medical diagnostic Time Factors Light Yersinia pestis Colony Count Microbial Immunoglobulin Variable Region Receptors Antigen B-Cell Biosensing Techniques Computational biology Antibodies Viral Escherichia coli O157 Transfection Sensitivity and Specificity Cell Line Encephalitis Virus Venezuelan Equine Aequorin medicine Pathogen B cell B-Lymphocytes Bacteriological Techniques Multidisciplinary Bacteria biology biology.organism_classification Antibodies Bacterial Rapid identification medicine.anatomical_structure Foot-and-Mouth Disease Virus Bacillus anthracis Viruses Biological warfare Identification (biology) Light emission
Zdroj:	Science. 301:213-215
ISSN:	1095-9203 0036-8075
DOI:	10.1126/science.1084920
Popis:	We report the use of genetically engineered cells in a pathogen identification sensor. This sensor uses B lymphocytes that have been engineered to emit light within seconds of exposure to specific bacteria and viruses. We demonstrated rapid screening of relevant samples and identification of a variety of pathogens at very low levels. Because of its speed, sensitivity, and specificity, this pathogen identification technology could prove useful for medical diagnostics, biowarfare defense, food- and water-quality monitoring, and other applications.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::81d7e05dccee0729594d98e3e4583017 https://doi.org/10.1126/science.1084920 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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