Prime editing efficiently generates W542L and S621I double mutations in two ALS genes in maize
| Autor: | Caixia Gao, Qi-Jun Chen, Qiupeng Lin, Yun Zhou, Yuan-Yuan Jiang, Xue-Chen Wang, Yi-Ping Chai, Xiu-Li Han, Min-Hui Lu, Yu Zhang, Qiang Zhang |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
lcsh:QH426-470 Genetic Vectors Short Report pegRNA Computational biology Biology Zea mays 01 natural sciences Prime (order theory) PE2 03 medical and health sciences Prime editing Genome editing Herbicide resistance CRISPR Gene CRISPR/Cas9 lcsh:QH301-705.5 030304 developmental biology Gene Editing 0303 health sciences Plants Genetically Modified Genetically modified rice Maize Acetolactate Synthase lcsh:Genetics lcsh:Biology (General) Mutagenesis Site-Directed ALS RNA Guide Kinetoplastida 010606 plant biology & botany |
| Zdroj: | Genome Biology, Vol 21, Iss 1, Pp 1-10 (2020) Genome Biology |
| DOI: | 10.1186/s13059-020-02170-5 |
| Popis: | Prime editing is a novel and universal CRISPR/Cas-derived precision genome-editing technology that has been recently developed. However, low efficiency of prime editing has been shown in transgenic rice lines. We hypothesize that enhancing pegRNA expression could improve prime-editing efficiency. In this report, we describe two strategies for enhancing pegRNA expression. We construct a prime editing vector harboring two pegRNA variants for W542L and S621I double mutations in ZmALS1 and ZmALS2. Compared with previous reports in rice, we achieve much higher prime-editing efficiency in maize. Our results are inspiring and provide a direction for the optimization of plant prime editors. |
| Databáze: | OpenAIRE |
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