Regulation of the biogenesis of OXPHOS complexes in cell transition from replicating to quiescent state

Autor: Loris Micelli, Sergio Papa, Giuliano Gattoni, Francesco Papa, Salvatore Scacco, Romina Ficarella, Anna Signorile, Arcangela Santeramo, Domenico De Rasmo
Rok vydání: 2014
Předmět:
Zdroj: Biochimica et Biophysica Acta (BBA) - Molecular Cell Research. 1843(4):675-684
ISSN: 0167-4889
DOI: 10.1016/j.bbamcr.2013.12.017
Popis: A study is presented on the expression of mitochondrial oxidative phosphorylation complexes in exponentially growing and serum-starved, quiescent human fibroblast cultures. The functional levels of respiratory complexes I and III and complex V (adenosine triphosphate (ATP) synthase) were found to be severely depressed in serum-starved fibroblasts. The depression of oxidative phosphorylation system (OXPHOS) complexes was associated with reduced levels of peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC-1α) and the down-stream nuclear respiratory factor 1 (NRF1) and mitochondrial transcription factors (TFAM). In serum-starved fibroblasts decrease of the catalytic activity of AMP cyclic dependent protein kinase (PKA) and phosphorylation of cAMP response element-binding protein (CREB), the transcription coactivator of the PGC-1α gene, was found. Hydroxytyrosol prevented the decline in the expression of the PGC-1α transcription cascade of OXPHOS complexes in serum-starved fibroblast cultures. The positive effect of HT was associated with activation of PKA and CREB phosphorylation. These results show involvement of PKA, CREB and PGC-1α in the regulation of OXPHOS in cell transition from the replicating to the quiescent state.
Databáze: OpenAIRE
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