Apoptosis-mediated neurotoxicity and altered gene expression induced by silver nanoparticles
| Autor: | Farzaneh Tafvizi, Maryam Ghooshchian, Parvin Khodarahmi |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
Male
0301 basic medicine Silver Health Toxicology and Mutagenesis medicine.medical_treatment Intraperitoneal injection Gene Expression Metal Nanoparticles Apoptosis Hippocampal formation Toxicology medicine.disease_cause Hippocampus Polymerase Chain Reaction 03 medical and health sciences Gene expression In Situ Nick-End Labeling medicine Animals Rats Wistar bcl-2-Associated X Protein TUNEL assay Chemistry Public Health Environmental and Occupational Health Neurotoxicity medicine.disease Molecular biology Rats 030104 developmental biology Real-time polymerase chain reaction Proto-Oncogene Proteins c-bcl-2 Oxidative stress |
| Zdroj: | Toxicology and Industrial Health. 33:757-764 |
| ISSN: | 1477-0393 0748-2337 |
| DOI: | 10.1177/0748233717719195 |
| Popis: | Although not fully recognized, the neurotoxic effects of silver nanoparticles (Ag-NPs) are thought to occur through induction of oxidative stress and apoptosis. To investigate the exact underlying molecular mechanism, we aimed to explore the apoptotic effects of intraperitoneal injection of Ag-NPs and investigated the possible attributed changes in the mRNA expression of Bcl-2 and Bax genes in the rat hippocampus. Two in vivo sets of experiments, one to demonstrate apoptosis and the other to assess gene expression, were conducted on male Wistar rats. In each set, the first group, acting as control, received saline and the other three groups received Ag-NP at doses of 100, 200, and 400 ppm for five successive days. Ten days after the last injection, hippocampal tissue of the first set of rats was assessed for apoptosis using terminal deoxynucleotidyl transferase-mediated deoxy uridine triphosphate nick-end labeling staining. In the second set of experiments, mRNA expression of Bcl-2 and Bax genes was evaluated using real-time polymerase chain reaction. Ag-NP treatment was shown to induce apoptosis in a dose-dependent manner. Furthermore, Ag-NP reduced mRNA level of Bcl-2 in the rat hippocampal cells at all investigated doses compared to the control group ( p < 0.001). The mRNA level of Bax, on the other hand, was increased in these cells. The increase was significant compared to the control group at the doses of 200 ppm ( p < 0.05) and 400 ppm ( p < 0.001). Our results show that Ag-NPs reduce Bcl-2 and increase Bax genes expression, resulting in increased Bax/Bcl-2 ratios in rat hippocampal cells. This altered gene expression induces cell apoptosis and contributes to the neurotoxicity of Ag-NPs. |
| Databáze: | OpenAIRE |
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