Effects of eye drops of Buddleja officinalis Maxim. extract on lacrimal gland cell apoptosis in castrated rats with dry eye
| Autor: | Wu Ql, Qing-Hua Peng, Tan Hy, Zhang, Yao Xl |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
Male
medicine.medical_treatment Untreated group Apoptosis Lacrimal gland Pharmacology Male rats medicine Animals Orchiectomy Rats Wistar Saline bcl-2-Associated X Protein biology Traditional medicine Lacrimal Apparatus Epithelial Cells biology.organism_classification Rats medicine.anatomical_structure Proto-Oncogene Proteins c-bcl-2 Complementary and alternative medicine Immunohistochemistry Dry Eye Syndromes Ophthalmic Solutions Buddleja officinalis Buddleja |
| Zdroj: | Journal of Chinese Integrative Medicine. 8:244-249 |
| ISSN: | 1672-1977 |
| DOI: | 10.3736/jcim20100308 |
| Popis: | Objective To explore the possible mechanism of eye drops of Buddleja officinalis extract in treating dry eye of castrated rats by analyzing the expressions of Bax and Bcl-2 proteins. Methods Forty-five Wistar male rats were randomly divided into sham-operated group, untreated group and eye drops of Buddleja officinalis Maxim. extract (treatment) group. The dry eye model was established with orchiectomy in the untreated group and treatment group. Rats in the treatment group were treated with eye drops of Buddleja officinalis Maxim. extract, one drop once, three times daily. Eyes of rats in the sham-operated group and untreated group were instilled with normal saline. After one-, two-, or three-month treatment, five rats in each group were scarified respectively. Then samples were taken to detect related indices. Expressions of Bax and Bcl-2 of lacrimal gland were checked by immunohistochemical method and quantity of apoptotic cells was counted. Results After one-, two- or three-month treatment, the quantities of expressions of Bax in acinar epithelial cells and glandular tube cells were significantly lower, and those of Bcl-2 were significantly higher in the treatment group than in the untreated group, and the quantities of apoptotic cells of the treatment group were significantly lower than those of the untreated group (P Conclusion The main components of extract of Buddleja officinalis Maxim. are flavonoids, which can significantly inhibit cell apoptosis in lacrimal gland. |
| Databáze: | OpenAIRE |
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