Performance of Oncomine Fusion Transcript kit for formalin‐fixed, paraffin‐embedded lung cancer specimens
| Autor: | Toshitaka Nagao, Jin Katayama, Jun Matsubayashi, Kazuko Sakai, Tatsuo Ohira, Norihiko Ikeda, Kazuto Nishio, Emi Iwamatsu, Akihiko Ito, Azusa Yoneshige, Tetsuya Mitsudomi |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
non‐small cell lung cancer
0301 basic medicine Cancer Research Lung Neoplasms Tissue Fixation Oncogene Proteins Fusion H&E stain Biology medicine.disease_cause Specimen Handling Fusion gene 03 medical and health sciences 0302 clinical medicine FISH Formaldehyde hemic and lymphatic diseases Biomarkers Tumor medicine ROS1 Humans Anaplastic Lymphoma Kinase Lung cancer Genetics Genomics and Proteomics companion diagnosis In Situ Hybridization Fluorescence Paraffin Embedding Reproducibility of Results Original Articles General Medicine medicine.disease ALK fusion gene next‐generation sequencer 030104 developmental biology Oncology Fusion transcript 030220 oncology & carcinogenesis Mutation Cancer research Adenocarcinoma Macrodissection Original Article Carcinogenesis |
| Zdroj: | Cancer Science |
| ISSN: | 1349-7006 1347-9032 |
| DOI: | 10.1111/cas.14016 |
| Popis: | Gene fusions play an important role in the carcinogenesis of lung adenocarcinoma. The recent association of four oncogenic driver genes, ALK, ROS1, RET, and NTRK1, as lung tumor predictive biomarkers has increased the need for precision medicine. We used formalin‐fixed, paraffin‐embedded tissue samples of non‐small cell lung cancer from 150 EGFR mutation‐negative cases and 10 fusion status‐known cases and compared the performance of the Oncomine Dx Fusion Transcript Test (ODxFT) with FISH break‐apart for the detection of ALK, RET, and ROS1 fusion genes. RNA was extracted from the paraffin‐embedded tissue samples with or without macrodissection under hematoxylin and eosin staining, and the ALK fusion gene was independently determined using these assays. Fusion detection analyses were successfully carried out using ODxFT in 150 cases, with only one invalid case. ALK fusion genes were detected at a frequency of 7.3% (11/150) in the lung cancer specimens. Concordance rate between the ODxFT and ALK‐FISH analyses was 99.3% (148/149). Sensitivity and specificity were 91.7% and 99.3%, respectively. All the samples with a known fusion status were accurately matched between the two assays. Our results show a high concordance rate between the ODxFT and ALK‐FISH analyses. ODxFT was thus validated as an effective method for detecting clinically significant ALK fusion genes in paraffin‐embedded tissue samples. |
| Databáze: | OpenAIRE |
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