Modulation of C3 gene expression in HepG2 human hepatoma cells
| Autor: | Naomi J. Sund, Marianne S. Wright, Tore G. Abrahamsen |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
Lipopolysaccharides
Regulation of gene expression Messenger RNA Carcinoma Hepatocellular Lipopolysaccharide Interleukin-6 Liver Neoplasms Immunology Acute-phase protein Inflammation Complement C3 Complement factor I Biology Molecular biology chemistry.chemical_compound Real-time polymerase chain reaction Gene Expression Regulation chemistry Gene expression Tumor Cells Cultured medicine Humans Immunology and Allergy medicine.symptom |
| Zdroj: | Immunology Letters. 76:119-123 |
| ISSN: | 0165-2478 |
| DOI: | 10.1016/s0165-2478(01)00180-8 |
| Popis: | Inflammation elicits an acute phase response, which includes changes in plasma concentrations of a number of cytokines, reflecting changes in their gene transcription in the liver. In this study, the induction of complement factor 3 (C3) was investigated in HepG2 cells, a human hepatoma cell line often used as a model system for cytokine-dependent expression of acute phase proteins of the liver. By using a very sensitive RT-PCR assay, the amount of mRNA for C3 was measured after induction with lipopolysaccharide (LPS) and interleukin-6 (IL-6). Both substances were found to up-regulate C3 gene expression. C3 mRNA level was lower in LPS-treated cells compared to IL-6 induction and also reached maximum expression at an earlier time point. These findings suggest a coordinate stimulation of C3 expression in the hepatocytes, which then maintains the host response to infectious agents. |
| Databáze: | OpenAIRE |
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