Silencing of FOXA2 decreases E‐cadherin expression and is associated with lymph node metastasis in oral cancer
| Autor: | Yuk-Kwan Chen, Yen-Yun Wang, Ling-Yi Xiao, Shyng-Shiou F. Yuan, Ruei-Nian Li, Yung-Ding Bow, Chang-Wei Su, Ching-Wei Hsu |
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| Rok vydání: | 2020 |
| Předmět: |
CDH1
Metastasis Gene product 03 medical and health sciences 0302 clinical medicine Antigens CD Cell Movement medicine Humans Gene silencing Gene Silencing Promoter Regions Genetic General Dentistry reproductive and urinary physiology biology business.industry Cancer Cell migration 030206 dentistry DNA Methylation respiratory system Cadherins medicine.disease Otorhinolaryngology Lymphatic Metastasis 030220 oncology & carcinogenesis embryonic structures Cancer cell DNA methylation Hepatocyte Nuclear Factor 3-beta Cancer research biology.protein Mouth Neoplasms Lymph Nodes business |
| Zdroj: | Oral Diseases. 26:756-765 |
| ISSN: | 1601-0825 1354-523X |
| Popis: | Objectives FOXA2 gene methylation links to the progression of cancers, but has not been documented in oral cancer. Herein, we explore the role of FOXA2 in the migration of oral cancer cells. Material and methods Methylation-specific PCR was applied for gene methylation. Wound healing and transwell experiments were tested for cell migration. FOXA2 expression in oral cancer tissues was addressed by immunohistochemistry, followed by statistical analysis of its association with clinical manifestations and patient survival. Results FOXA2 bound to the promoter of CDH1 and enhanced the expression of its gene product E-cadherin, and decreased the cancer cell migration activity. High FOXA2 expression in oral cancer tissues was associated with high E-cadherin expression, decreased lymph node metastasis, and increased patient survival. Conclusion FOXA2-E-cadherin link is involved in regulation of oral cancer cell metastasis and provides a new insight for the tumor suppressor activity of FOXA2 in oral cancer. |
| Databáze: | OpenAIRE |
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