Determination of antibacterial activity and metabolite profile of Ruta graveolens against Streptococcus mutans and Streptococcus sobrinus
| Autor: | Ramasamy Senthilkumar, Hamzah Abdulrahman Salman, Sankarasetty Venkatesh, Aamer Mousa Ali, B.S. Gnanesh Kumar |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
food.ingredient Metabolite Ruta graveolens 030231 tropical medicine 030106 microbiology lcsh:Medicine Streptococcus sobrinus 03 medical and health sciences chemistry.chemical_compound electrospray ionization-tandem mass spectrometry 0302 clinical medicine food γ-fagarine Agar anticariogenic biology Traditional medicine Chemistry lcsh:R mutans streptococci biology.organism_classification Streptococcus mutans kokusaginine stomatognathic diseases Kokusaginine dental caries Antibacterial activity Bacteria |
| Zdroj: | Journal of Laboratory Physicians, Vol 10, Iss 03, Pp 320-325 (2018) |
| ISSN: | 0974-7826 0974-2727 |
| DOI: | 10.4103/JLP.JLP_160_17 |
| Popis: | BACKGROUND: Ruta graveolens is one of the most used phytomedicines. To date, there is no report of determining the bioactivity of R. graveolens against cariogenic causing bacteria (Streptococcus mutans and Streptococcus sobrinus). OBJECTIVE: The objective of the present study was to determine the antibacterial activity and metabolite profile of R. graveolens against S. mutans and S. sobrinus. MATERIAL AND METHODS: R. graveolens plant material was collected and processed in the month of February. The plant material was extracted by Soxhlet apparatus using methanol solvent. Two strains of S. mutans and two strains of S. sobrinus were isolated from dental caries-active participants and cultured on mitis salivarius-bacitracin agar. The antibacterial susceptibility testing of methanolic extract of R. graveolens was performed by disc diffusion method. The metabolite profile of the plant extract was determined using electrospray ionization-tandem mass spectrometry. RESULTS: The methanolic extract of R. graveolens showed a promising antibacterial activity against S. mutans and S. sobrinus. Two compounds named γ-fagarine and kokusaginine were identified from the methanolic extract of R. graveolens. CONCLUSIONS: The study concluded that R. graveolens contains significant antibacterial activity. However, further investigations are suggested to understand the anticaries properties of these pure compounds. |
| Databáze: | OpenAIRE |
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