Evaluation of the VIDAS glutamate dehydrogenase assay for the detection of Clostridium difficile
| Autor: | Eun Joo Lee, Bo-Moon Shin, Seon Yeong Lee, Jung Wha Moon |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Botulinum Toxins 030106 microbiology Gene Expression Enzyme-Linked Immunosorbent Assay Biology medicine.disease_cause Polymerase Chain Reaction Sensitivity and Specificity Microbiology law.invention Feces 03 medical and health sciences 0302 clinical medicine Bacterial Proteins Glutamate Dehydrogenase Predictive Value of Tests law medicine Humans 030212 general & internal medicine Enterocolitis Pseudomembranous Polymerase chain reaction Clostridioides difficile Toxin Glutamate dehydrogenase Clostridium difficile Molecular biology Predictive value Infectious Diseases FLUORESCENT IMMUNOASSAY |
| Zdroj: | Anaerobe. 40:68-72 |
| ISSN: | 1075-9964 |
| DOI: | 10.1016/j.anaerobe.2016.06.001 |
| Popis: | We evaluated the performance of the VIDAS GDH assay for the detection of Clostridium difficile. In total, 350 fecal specimens collected from patients clinically suspected of having CDI were analyzed by C. difficile culture and enzyme-linked fluorescent immunoassay (VIDAS GDH); the results were compared with those of toxigenic C. difficile culture (TC), PCR (Xpert C. difficile assay), and toxin AB EIA (VIDAS CDAB). The numbers of culture-positive and culture-negative samples were 108 and 242, respectively. The concordance between the GDH assay and C. difficile culture was 90.3%. With PCR, 12 more samples were found to be positive in GDH-positive/C. difficile culture-negative specimens. Thus, the concordance between GDH assay and C. difficile culture/PCR was 93.7%. The sensitivity, specificity, positive predictive value, and negative predictive value of the VIDAS GDH assay were 97.2%, 87.2%, 77.2%, and 98.6%, respectively, based on the C. difficile culture, and 97.5%, 91.7%, 86.0%, and 98.6%, respectively, based on C. difficile culture/PCR. Positivity rates of the GDH assay were partially associated with those of semi-quantitative C. difficile cultures, which were maximized in grade 3 (>100 colony-forming unit [CFU]) compared with grade 1 ( We evaluated the two-step or three-step algorithm using GDH assay as a first step. No toxin EIA-positive case was found among GDH-negative samples, and 60.8% (48/79) were TC- and/or PCR-positive among the GDH-positive/toxin EIA-negative samples. Thus, approximately 25% of the 350 samples required a confirmatory test (TC or PCR) in the GDH-toxin EIA algorithm, whereas only 2.3% of the total samples in GDH-PCR algorithm was discrepant and required another confirmatory test like TC. |
| Databáze: | OpenAIRE |
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