Determination of chlorogenic acids and caffeine in homemade brewed coffee prepared under various conditions
| Autor: | Se-Ra Hong, Moon-Seog Oh, A. M. Abd El-Aty, Jong-Sup Jeon, Kwang-Hee Park, Jae-Han Shim, Han-Taek Kim, Il-Hyung Jeong |
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| Rok vydání: | 2017 |
| Předmět: |
Clinical Biochemistry
Coffee 01 natural sciences Biochemistry High-performance liquid chromatography Analytical Chemistry chemistry.chemical_compound 0404 agricultural biotechnology Chlorogenic acid Limit of Detection Caffeine Food science Particle Size Green coffee Chromatography High Pressure Liquid Roasting Chromatography Neochlorogenic acid business.industry Chemistry 010401 analytical chemistry Temperature Reproducibility of Results 04 agricultural and veterinary sciences Cell Biology General Medicine 040401 food science 0104 chemical sciences Linear Models Brewing Chlorogenic Acid business |
| Zdroj: | Journal of Chromatography B. 1064:115-123 |
| ISSN: | 1570-0232 |
| DOI: | 10.1016/j.jchromb.2017.08.041 |
| Popis: | Coffee, a complex mixture of more than 800 volatile compounds, is one of the most valuable commodity in the world, whereas caffeine and chlorogenic acids (CGAs) are the most common compounds. CGAs are mainly composed of caffeoylquinic acids (CQAs), dicaffeoylquinic acids (diCQAs), and feruloylquinic acids (FQAs). The major CGAs in coffee are neochlorogenic acid (3-CQA), cryptochlorogenic acid (4-CQA), and chlorogenic acid (5-CQA). Many studies have shown that it is possible to separate the isomers of FQAs by high-performance liquid chromatography (HPLC). However, some authors have shown that it is not possible to separate 4-feruloylquinic acid (4-FQA) and 5-feruloylquinic acid (5-FQA) by HPLC. Therefore, the present study was designated to investigate the chromatographic problems in the determination of CGAs (seven isomers) and caffeine using HPLC-DAD. The values of determination coefficient (R2) calculated from external-standard calibration curves were >0.998. The recovery rates conducted at 3 spiking levels ranged from 99.4% to 106.5% for the CGAs and from 98.8% to 107.1% for the caffeine. The precision values (expressed as relative standard deviations (RSDs)) were |
| Databáze: | OpenAIRE |
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