Magnetic Resonance Detection of CD34+ Cells from Umbilical Cord Blood Using a 19F Label
| Autor: | Mariette N. D. ter Borg, Eric Braakman, Sandra T. van Tiel, Jan J. Cornelissen, Joost C. Haeck, Bernard J. M. van Rossum, Elwin J. C. Rombouts, Ramon M. van der Werf, Lucia E. Duinhouwer, Gabriela N. Doeswijk, Monique R. Bernsen, Gyula Kotek |
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| Přispěvatelé: | Hematology, Erasmus MC other, Radiology & Nuclear Medicine |
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Fluorine Radioisotopes
Pathology medicine.medical_specialty Magnetic Resonance Spectroscopy Time Factors medicine.medical_treatment CD34 lcsh:Medicine Antigens CD34 Hematopoietic stem cell transplantation Biology Umbilical cord Colony-Forming Units Assay Polylactic Acid-Polyglycolic Acid Copolymer In vivo medicine Humans Lactic Acid lcsh:Science Cells Cultured Microscopy Confocal Multidisciplinary lcsh:R Reproducibility of Results Fetal Blood Flow Cytometry Hematopoietic Stem Cells Transplantation Haematopoiesis medicine.anatomical_structure surgical procedures operative Cell Tracking Cancer research Nanoparticles lcsh:Q Stem cell Polyglycolic Acid Research Article Homing (hematopoietic) |
| Zdroj: | PLoS ONE, Vol 10, Iss 9, p e0138572 (2015) PLoS One (print), 10(9). Public Library of Science BASE-Bielefeld Academic Search Engine PLoS ONE |
| ISSN: | 1932-6203 |
| Popis: | Impaired homing and delayed recovery upon hematopoietic stem cell transplantation (HSCT) with hematopoietic stem cells (HSC) derived from umbilical cord blood (UCB) is a major problem. Tracking transplanted cells in vivo will be helpful to detect impaired homing at an early stage and allows early interventions to improve engraftment and outcome after transplantation. In this study, we show sufficient intracellular labeling of UCB-derived CD34(+) cells, with F-19-containing PLGA nanoparticles which were detectable with both flow cytometry and magnetic resonance spectroscopy (MRS). In addition, labeled CD34(+) cells maintain their capacity to proliferate and differentiate, which is pivotal for successful engraftment after transplantation in vivo. These results set the stage for in vivo tracking experiments, through which the homing efficiency of transplanted cells can be studied. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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