Evaluation of an in vitro hsp70 induction test for toxicity assessment of complex mixtures: comparison with chemical analyses and ecotoxicity tests
| Autor: | Hélène Magaud, Jean-Marc Porcher, Selim Ait-Aissa, Pascal Pandard, Eric Thybaud, André-Patrick Arrigo |
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| Rok vydání: | 2003 |
| Předmět: |
Chloramphenicol O-Acetyltransferase
Health Toxicology and Mutagenesis Cell Culture Techniques Industrial Waste Plant Development Daphnia Toxicology Chloramphenicol acetyltransferase chemistry.chemical_compound Genes Reporter Toxicity Tests Ecotoxicology Animals Humans Drug Interactions HSP70 Heat-Shock Proteins Oligochaeta Promoter Regions Genetic Reporter gene biology Bacteria Dose-Response Relationship Drug Public Health Environmental and Occupational Health Ceriodaphnia dubia Eukaryota General Medicine Models Theoretical biology.organism_classification Pollution Biochemistry chemistry Toxicity Luminescent Measurements Ecotoxicity Growth inhibition HeLa Cells |
| Zdroj: | Ecotoxicology and environmental safety. 54(1) |
| ISSN: | 0147-6513 |
| Popis: | The aim of this study was to assess the potential of a human cell line containing the hsp70 promoter linked to the chloramphenicol acetyltransferase reporter gene in evaluating the toxic potential of complex mixtures. Cells were exposed to eluates of industrial wastes and the cellular responses were compared with the metal contents of the samples and with standardized aquatic (microalgal growth inhibition, daphnia Immobilization, bacterial luminescence inhibition, Ceriodaphnia dubia reproduction inhibition) and terrestrial (earthworm lethality, plant growth inhibition) tests. The hsp70 promoter was significantly induced by 11 of 14 samples, with different dose-response patterns. Significant correlations of in vitro induction potency with aquatic ecotoxicity, especially with chronic tests, and with the metal contents of the samples were observed. Our study provides new information on the relevance of hsp70 gene induction as a criterion of toxicity and suggests its usefulness for the detection of toxicity associated with metallic pollution in complex mixtures. |
| Databáze: | OpenAIRE |
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