Centrifugal partition chromatography enables selective enrichment of trimeric and tetrameric proanthocyanidins for biomaterial development
| Autor: | Guido F. Pauli, RS Phansalkar, Shao-Nong Chen, Joo Won Nam, Ana K. Bedran-Russo, A.A. Leme, Berdan Aydin, James B. McAlpine |
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| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
Separation scheme
Centrifugal partition chromatography Trimer Biocompatible Materials Hard tissue 01 natural sciences Biochemistry Article Analytical Chemistry Proanthocyanidins Chromatography High Pressure Liquid Chromatography 010405 organic chemistry Chemistry Plant Extracts 010401 analytical chemistry Organic Chemistry Biomaterial food and beverages Reproducibility of Results General Medicine humanities 0104 chemical sciences Proanthocyanidin Polyphenol Cinnamon Bark Chromatography Liquid |
| Popis: | Proanthocyanidins (PACs) find wide applications for human use including food, cosmetics, dietary supplements, and pharmaceuticals. The chemical complexity associated with PACs has triggered the development of various chromatographic techniques, with countercurrent separation (CCS) gaining in popularity. This study applied the recently developed DESIGNER (Depletion and Enrichment of Select Ingredients Generating Normalized Extract Resources) approach for the selective enrichment of trimeric and tetrameric PACs using centrifugal partition chromatography (CPC). This CPC method aims at developing PAC based biomaterials, particularly for their application in restoring and repairing dental hard tissue. A general separation scheme beginning with the depletion of polymeric PACs, followed by the removal of monomeric flavan-3-ols and a final enrichment step produced PAC trimer and tetramer enriched fractions. A successful application of this separation scheme is demonstrated for four polyphenol rich plant sources: grape seeds, pine bark, cinnamon bark, and cocoa seeds. Minor modifications to the generic DESIGNER CCS method were sufficient to accommodate the varying chemical complexities of the individual source materials. The step-wise enrichment of PAC trimers and tetramers was monitored using normal phase TLC and Diol-HPLC-UV analyses. CPC proved to be a reliable tool for the selective enrichment of medium size oligomeric PACs (OPACs). This method plays a key role in the development of dental biomaterials considering its reliability and reproducibility, as well as its scale-up capabilities for possible larger-scale manufacturing. (C) 2017 Elsevier B.V. All rights reserved. |
| Databáze: | OpenAIRE |
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