ON THE MECHANISM OF STIMULATION OF AMP-AMINOHYDROLASE ACTIVITY BY HEXOKINASE IN BRAIN TISSUE
| Autor: | A. V. Haroutunian, H. Ch. Buniatian |
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| Rok vydání: | 1974 |
| Předmět: |
Time Factors
Swine Guinea Pigs Stimulation Saccharomyces cerevisiae Brain tissue Biology Biochemistry Isozyme Chromatography DEAE-Cellulose Cellular and Molecular Neuroscience chemistry.chemical_compound Cytosol Species Specificity Aminohydrolases Ammonia Hexokinase Animals Urea chemistry.chemical_classification Brain Molecular biology Adenosine Monophosphate Yeast Rats Molecular Weight Kinetics Enzyme chemistry Sephadex Chromatography Gel Cattle Spectrophotometry Ultraviolet Rabbits |
| Zdroj: | Journal of Neurochemistry. 23:297-302 |
| ISSN: | 1471-4159 0022-3042 |
| DOI: | 10.1111/j.1471-4159.1974.tb04357.x |
| Popis: | —A hexokinase has been isolated from brain tissue on Sephadex G-100 and DEAE cellulose which is similar to yeast enzyme in stimulating the AMP-aminohydrolase activity of rat brain soluble fractions. This effect of hexokinase is influenced neither by N-acetyl-glucosamine nor noradrenaline. An isoenzyme of hexokinase isolated from brain tissue on DEAE cellulose, having properties similar to that of the muscle enzyme, has no effect on AMP-aminohydrolase activity. The activating effect of yeast hexokinase is not due to its oligomeric structure. Enzyme subunits obtained by the treatment of native yeast enzyme by urea also activate AMP-aminohydrolase of rat brain soluble fractions. |
| Databáze: | OpenAIRE |
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