Perturbed Angular Correlation Study of a Haptenic Molecule

Autor:	John D. Baldeschwieler, M. W. Sundberg, Claude F. Meares
Rok vydání:	1972
Předmět:	Rabbit Antibody Multidisciplinary Nanotechnology Quantitative Biology::Other chemistry.chemical_compound chemistry Physical Sciences: Chemistry Angular correlation Good evidence Physical chemistry Molecule Dinitrophenyl Chelation Caltech Library Services Macromolecule
Zdroj:	Proceedings of the National Academy of Sciences. 69:3718-3722
ISSN:	1091-6490 0027-8424
DOI:	10.1073/pnas.69.12.3718
Popis:	The angular correlation of the 173-247 keV gamma-ray cascade after the electron-capture decay of 111 In is strongly perturbed when the 1- p -nitrophenyl-ethylenediaminetetraacetate chelate of 111 In 8+ is added to a solution containing rabbit antibody to dinitrophenyl groups. The radioactive chelate can be displaced by the addition of dinitrophenyllysine or unlabeled chelate. The average association constant between the antibody and the labeled chelate has been estimated from perturbed angular correlation measurements; this value is compared to the results of equilibrium dialysis. These experiments provide good evidence that information concerning macromolecular behavior can be obtained from perturbed angular correlation experiments that use chemically specific labels.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::81bea34c885c301e80ab116de04175ec https://doi.org/10.1073/pnas.69.12.3718 Zobrazit plný text záznamu