Conjugation of hyaluronan to proteins
| Autor: | Monica Campisi, Matteo Pasqualin, Davide Renier, Anna Mero, Gianfranco Pasut |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Erythrocytes
Polymers and Plastics RNase P medicine.medical_treatment Peptide chemistry.chemical_compound Materials Testing Hyaluronic acid Materials Chemistry medicine Animals Insulin Trypsin Hyaluronic Acid chemistry.chemical_classification Aldehydes Immunogenicity Organic Chemistry Proteins Ribonuclease Pancreatic Rats Enzyme chemistry Biochemistry Cattle medicine.drug Conjugate |
| Zdroj: | Carbohydrate Polymers. 92:2163-2170 |
| ISSN: | 0144-8617 |
| DOI: | 10.1016/j.carbpol.2012.11.090 |
| Popis: | Polymer conjugation has been widely exploited to prolong half-life and reduce immunogenicity of therapeutic proteins. Here, the potentials of hyaluronic acid (HA) have been investigated by studying the conjugates with two model enzymes, trypsin and RNase A, and with insulin. As the direct coupling of proteins to the HA's carboxylic groups can cause cross-linking problems, a hyaluronan-aldehyde derivative has been synthesized for N-terminal site-selective conjugation. HA conjugation, termed HAylation, preserved the activities of enzymes and their thermal stabilities. Insulin HAylation was studied by preparing two conjugates with different peptide loadings (32% and 17%, w/w). Noticeably, the conjugate with the lower loading showed the greater effect on blood glucose level. The 17% HA-insulin conjugate showed a lowering effect on blood glucose level for up to 6h, while free insulin exhausted its action after 1h. This study highlights the potentials of hyaluronan-aldehyde for protein delivery. |
| Databáze: | OpenAIRE |
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