Ru(ii )-polypyridyl surface functionalised gold nanoparticles as DNA targeting supramolecular structures and luminescent cellular imaging agents
| Autor: | Thorfinnur Gunnlaugsson, Robert B. P. Elmes, Bjørn la Cour Poulsen, Miguel Martínez-Calvo, Kim N. Orange, D. Clive Williams |
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| Rok vydání: | 2016 |
| Předmět: |
Hydrodynamic radius
Confocal Supramolecular chemistry Metal Nanoparticles Nanotechnology 02 engineering and technology 010402 general chemistry 01 natural sciences Ruthenium chemistry.chemical_compound Neoplasms Humans General Materials Science Optical Imaging DNA Neoplasm 021001 nanoscience & nanotechnology Fluorescence 0104 chemical sciences chemistry Transmission electron microscopy Colloidal gold Gold 0210 nano-technology Luminescence DNA HeLa Cells |
| Zdroj: | Nanoscale. 8:563-574 |
| ISSN: | 2040-3372 2040-3364 |
| Popis: | The development of Ru(II) functionalized gold nanoparticles 1–3·AuNP is described. These systems were found to be mono-disperse with a hydrodynamic radius of ca. 15 nm in water but gave rise to the formation of higher order structures in buffered solution. The interaction of 1–3·AuNP with DNA was also studied by spectroscopic and microscopic methods and suggested the formation of large self-assembly structures in solution. The uptake of 1–3·AuNP by cancer cells was studied using both confocal fluorescence as well as transmission electron microscopy (TEM), with the aim of investigating their potential as tools for cellular biology. These systems displaying a non-toxic profile with favourable photophysical properties may have application across various biological fields including diagnostics and therapeutics. |
| Databáze: | OpenAIRE |
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