Induction of angiogenesis and neural progenitor cells by basic fibroblast growth factor‐releasing polyglycolic acid sheet following focal cerebral infarction in mice

Autor: Yoshiro Ito, Ayako Oyane, Mayu Yasunaga, Koji Hirata, Motohiro Hirose, Hideo Tsurushima, Yuzuru Ito, Yuji Matsumaru, Eiichi Ishikawa
Rok vydání: 2022
Předmět:
Zdroj: Journal of Biomedical Materials Research Part A. 110:1964-1975
ISSN: 1552-4965
1549-3296
DOI: 10.1002/jbm.a.37434
Popis: Biodegradable sheets loaded with basic fibroblast growth factor (bFGF) are prepared as novel bFGF-releasing systems from polyglycolic acid nonwoven fabric by oxygen plasma treatment followed by bFGF adsorption. In the present study, we investigated the therapeutic effects of this system on a focal cerebral infarction model (CB-17 mouse). A preliminary in vitro study showed that this system released bFGF in an acellular culture medium, thereby keeping the bFGF concentration in the medium at ≥5 ng/ml for a prolonged period of 7 days. The released bFGF from this system retained its biological activity to enhance endothelial tube formation in vitro. In a mouse model of subacute focal cerebral infarction, this system increased the expression of endogenous vascular endothelial growth factor in the peri-infarct cortex and subventricular zone, promoted angiogenesis in the striatum, and increased neural progenitor cells in the peri-infarct cortex. Thus, this bFGF-releasing system has the potential to be a novel therapeutic approach for cerebral infarction.
Databáze: OpenAIRE
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