Placental protein 14 as a potential biomarker for diagnosis of preterm premature rupture of membranes

Autor:	Jun Gao, Bin Zhou, Yanqin Li, Lin Zhang, Linbo Gao, Tao Wang, Yanyun Wang, Yujie Liang, Qiongli Yang, Haibo Luo, Guanglu Che
Rok vydání:	2018
Předmět:	Adult Cancer Research Fetal Membranes Premature Rupture Amniotic fluid Adolescent Prom Biochemistry Andrology 03 medical and health sciences Extracellular matrix protein 1 0302 clinical medicine Pregnancy Keratin Genetics Medicine Humans Mesothelin 030212 general & internal medicine liquid chromatography-tandem mass spectrometry education Molecular Biology chemistry.chemical_classification education.field_of_study 030219 obstetrics & reproductive medicine Zymogen granule protein 16 biology business.industry lateral flow assay Articles medicine.disease Oncology chemistry Glycodelin premature rupture of membranes Proteome placental protein 14 biology.protein Molecular Medicine biomarker Female business Premature rupture of membranes Biomarkers
Zdroj:	Molecular Medicine Reports
ISSN:	1791-3004
Popis:	Premature rupture of membranes (PROM) is a common pregnancy complication that frequently results in maternal and perinatal morbidity. The present methods for diagnosing PROM do not satisfy clinical requirements. The present study aimed to examine the proteome profile of amniotic fluid (AF) and maternal plasma, screen unique proteins in AF, and evaluate their diagnostic value for diagnosing PROM. The proteome profiles of AF and maternal plasma were examined via liquid chromatography coupled with tandem mass spectrometry‑based proteomic techniques. The protein expression levels of diagnostic candidates in AF, maternal plasma and vaginal fluid were determined by ELISA analysis and Magnetic Luminex® screening assays. The diagnostic value of potential biomarkers was evaluated using receiver operating characteristic curves. A lateral flow assay was developed based on colloidal gold immunochromatography technology. The present study identified 540 unique proteins in AF, 12 of which were chosen for further detection. The present results demonstrated that expression levels of pulmonary surfactant‑associated protein B, BPI fold‑containing family A member 1, zymogen granule protein 16 homolog B, EGF‑containing fibulin‑like extracellular matrix protein 1, keratin, type II cytoskeletal 4, keratin, type I cytoskeletal 19, placental protein 14 (PP14), insulin‑like growth factor‑binding protein 2, mesothelin and serpin family B member 3 were significantly higher in AF compared with in maternal plasma (P
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::81b5fc1a016eaaa76c46ec8486e6446f https://pubmed.ncbi.nlm.nih.gov/29749501 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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