Evaluation of FTA® cards as a laboratory and field sampling device for the detection of foot-and-mouth disease virus and serotyping by RT-PCR and real-time RT-PCR
| Autor: | Srinivasan Alwar Villuppanoor, Madhanmohan Muthukrishnan, Kumar Ralla, Nagendrakumar Balasubramanian Singanallur |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
Serotype
Aphthovirus Foot-and-mouth disease Clinical Laboratory Techniques Reverse Transcriptase Polymerase Chain Reaction viruses Biology biology.organism_classification medicine.disease Virology Sampling Studies Virus Sampling device Real-time polymerase chain reaction Foot-and-Mouth Disease Virus Foot-and-Mouth Disease Sample Size medicine Animals RNA Viral Typing Serotyping Foot-and-mouth disease virus |
| Zdroj: | Journal of Virological Methods. 151:311-316 |
| ISSN: | 0166-0934 |
| DOI: | 10.1016/j.jviromet.2008.05.020 |
| Popis: | Foot-and-mouth disease virus (FMDV) samples transported to the laboratory from far and inaccessible areas for serodiagnosis pose a major problem in a tropical country like India, where there is maximum temperature fluctuation. Inadequate storage methods lead to spoilage of FMDV samples collected from clinically positive animals in the field. Such samples are declared as non-typeable by the typing laboratories with the consequent loss of valuable epidemiological data. The present study evaluated the usefulness of FTA® Classic Cards for the collection, shipment, storage and identification of the FMDV genome by RT-PCR and real-time RT-PCR. The stability of the viral RNA, the absence of infectivity and ease of processing the sample for molecular methods make the FTA® cards a useful option for transport of FMDV genome for identification and serotyping. The method can be used routinely for FMDV research as it is economical and the cards can be transported easily in envelopes by regular document transport methods. Live virus cannot be isolated from samples collected in FTA® cards, which is a limitation. This property can be viewed as an advantage as it limits the risk of transmission of live virus. |
| Databáze: | OpenAIRE |
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