Natural nodular worm infection in goats induces eosinophil extracellular DNA trap (EET) formation
| Autor: | Abu Anas, S. S. Labony, Takeshi Hatta, Nurjahan Begum, Naotoshi Tsuji, Md. Abdul Alim, Anisuzzaman, Md. Mokbul Hossain, Md. Golam Yasin |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Male 030231 tropical medicine Granulocyte Biology Extracellular Traps Microbiology 03 medical and health sciences 0302 clinical medicine medicine Animals Large intestine Oesophagostomum columbianum Innate immune system Goat Diseases Goats 030108 mycology & parasitology Eosinophil Extracellular dna Immunity Innate Eosinophils Oesophagostomum Infectious Diseases medicine.anatomical_structure Parasitology Female Oesophagostomiasis Nodular worm |
| Zdroj: | Parasitology international. 79 |
| ISSN: | 1873-0329 |
| Popis: | Extracellular DNA Trap (ET) formation by granulocyte is a strong innate immune machinery that plays crucial roles in trapping and killing of pathogens. Here, we show Eosinophil Extracellular DNA Trap (EET) formation in goats naturally infected with nodular worms (Oesophagostomum columbianum, Strongyloidae: Nematoda). By a slaughterhouse based survey, we found that 60% goats were infected with nodular worms. We detected numerous, hard and pale yellow to dark black nodules of variable sizes (0.25-2 cm) in the large intestine and the number of nodules were significantly (p .05) higher in the cecum (21.7 ± 17.9) than in the colon (10.1 ± 9.9). Histologically, pink colored circumscribed caseous mass was surrounded by a dense zone of infiltration and fibrous proliferation along with massive infiltration of eosinophils in and around the necrotic mass. DAPI staining revealed huge accumulation of extracellular DNA, which formed wide ridge like structure surrounding the necrotic zone. Massive release of eosinophils cationic proteins (ECP), a helmintho-toxic substance, was found into the lesions. Collectively, our results suggest that nodular worm infection induces EETosis and ECP release, and is one of the major parasitic problem affecting Black Bengal goats that causes distortion of normal architecture of the gut wall. |
| Databáze: | OpenAIRE |
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