Induction of cytotoxic T lymphocytes in mice against the principal neutralizing domain of HIV-1 by immunization with an engineered T-cytotoxic-T-helper synthetic peptide construct
| Autor: | Pablo Sarobe, Juan José Lasarte, Francisco Borrás-Cuesta, Arturo Gullón, Jesús Prieto |
|---|---|
| Rok vydání: | 1992 |
| Předmět: |
Cellular immunity
HIV Antigens Surface Properties media_common.quotation_subject Molecular Sequence Data Immunology chemical and pharmacologic phenomena Biology HIV Antigens/immunology Epitope Mice Immune system T-Lymphocytes Helper-Inducer/immunology HIV-1/immunology Animals Cytotoxic T cell Amino Acid Sequence Internalization media_common Mice Inbred BALB C food and beverages T-Lymphocytes Helper-Inducer T lymphocyte T-Lymphocytes Cytotoxic/immunology Virology CTL Immunization Peptides/immunology HIV-1 Female Peptides Spleen T-Lymphocytes Cytotoxic |
| Zdroj: | Dadun. Depósito Académico Digital de la Universidad de Navarra instname |
| ISSN: | 0008-8749 |
| DOI: | 10.1016/0008-8749(92)90140-k |
| Popis: | Peptide constructs were engineered by colinear synthesis of two short synthetic peptide determinants: a determinant recognized by T helper cells (TD,) and a determinant recognized by T cytotoxic cells (TD,). Three types of constructs were synthesized: TD,-TDr, , TD,-TD, , and TD,,KK-TD,, where KK are two lysine residues. In vivo immunization with free construct induced cytolytic lymphocytes (CTL) only in the case of TD,-TD,,. However, immunization with spleen cells to which these constructs had been internalized by hypertonic shock, induced CTL activity in all three cases. No CTL could be induced after immunization with free TD, in either protocol. These results indicate that cell internalization of the construct might be essential for CTL induction, and also, that “help” from the TDr, seems to be required. o 1992 Academic PKSS, IX. |
| Databáze: | OpenAIRE |
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