Use of a Multifunctional Column for the Determination of Deoxynivalenol in Grains, Grain Products, and Processed Foods
| Autor: | Carolyn J Oles, Lei Bao, Chelsea Sapp, Mary W Trucksess, Kevin D. White |
|---|---|
| Rok vydání: | 2011 |
| Předmět: |
Fusarium
Flour High-performance liquid chromatography Mass Spectrometry Analytical Chemistry chemistry.chemical_compound Vomitoxin Tandem Mass Spectrometry Environmental Chemistry Food science Mycotoxin Chromatography High Pressure Liquid Triticum Pharmacology biology Bran Extraction (chemistry) Reproducibility of Results Hordeum Oryza Bread Repeatability Reference Standards Contamination biology.organism_classification Solutions chemistry Spectrophotometry Ultraviolet Edible Grain Trichothecenes Agronomy and Crop Science Food Analysis Food Science |
| Zdroj: | Journal of AOAC International. 94:1506-1512 |
| ISSN: | 1060-3271 |
| DOI: | 10.5740/jaoacint.10-477 |
| Popis: | Deoxynivalenol (DON), also known as vomitoxin, belongs to a class of naturally occurring mycotoxins produced by Fusarium spp. DON, 12, 13-epoxy-3,7 trihydroxytrichothec-9-en-8-one, is one of the most frequently detected mycotoxins in agricultural commodities worldwide. A method consisting of extraction, filtration, column cleanup, and RP-HPLC-UV separation and quantitation was validated for the determination of DON in grains (rice and barley), grain products (whole wheat flour, white flour, wheat germ, and wheat bran), and processed foods (bread, breakfast cereals, and pretzels). A 25 g test portion was extracted with 100 mL acetonitrile-water (84 + 16, v/v). After blending for 3 min, the supernatant was applied to a multifunctional column (MycoSep 225). The purified filtrate (2 mL) was evaporated to dryness and redissolved in the mobile phase. The toxins were then subjected to RP-HPLC-UV analysis. The accuracy and repeatability characteristics of the method were determined. Recoveries of DON added at levels ranging from 0.5 to 1.5 microg/g for all test matrixes were from 75 to 98%. SD and RSD(r) ranged from 0.7 to 11.6% and 0.9 to 12.7%, respectively. Within-laboratory HorRat values were from 0.1 to 0.7 for all matrixes analyzed. The method was found to meet AOAC method performance criteria for grains, grain products, and processed foods. The identity of DON in naturally contaminated test sample extracts was confirmed by HPLC/MS/MS analysis. |
| Databáze: | OpenAIRE |
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