Argyrophilic nucleolar organizing region associated protein synthesis for cytologic discrimination of follicular thyroid lesions
| Autor: | Murat Oktay, Anzel Bahadir, Nurhan Cücer, N A Oktay, R Eroz, Havva Erdem, Feyza Başar, Lütfi Akyol |
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| Rok vydání: | 2014 |
| Předmět: |
Adenoma
Adult Male Silver Staining Pathology medicine.medical_specialty Histology Adolescent follicular lesions Carcinoma Papillary Follicular thyroid Thyroid carcinoma Young Adult Biopsy Nucleolus Organizer Region Carcinoma medicine Humans Thyroid Neoplasms Aged AgNORs medicine.diagnostic_test business.industry Thyroid Antigens Nuclear General Medicine Middle Aged medicine.disease Medical Laboratory Technology Fine-needle aspiration medicine.anatomical_structure Cytopathology Protein Biosynthesis cytology Female Nucleolus organizer region business |
| Zdroj: | Biotechnic & Histochemistry. 90:179-183 |
| ISSN: | 1473-7760 1052-0295 0003-5211 |
| Popis: | WOS: 000352110200003 PubMed: 25420891 Fine needle aspiration biopsy (FNAB) of the thyroid gland is an important tool for preoperative diagnosis; however, its benefit is limited for follicular lesions. Nucleolar organizer regions (NORs) are ribosomal gene regions that stain with silver (Ag) when they are active. These regions can be used to differentiate neoplastic and non-neoplastic lesions. We used a new AgNOR technique to investigate FNAB of cases diagnosed as follicular adenoma and carcinoma. Fourteen cases of follicular thyroid carcinoma (FTC) and 28 cases of thyroid follicular adenomas (FA) were stained using the silver NOR-associated protein (AgNOR) technique. One hundred nuclei per sample were examined, AgNORs were counted, and the total AgNOR area/nuclear area (TNORa/Na) ratio of each cell was calculated. We found that cases with FTC had significantly higher TNORa/Na than cases of FA. Also, cases with FTC had significantly higher AgNOR counts than cases with FA. AgNOR counting may help discriminate FTC and FA by routine cytopathology before surgery. |
| Databáze: | OpenAIRE |
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