AgNOR counts and their combination with flow cytometric analyses and clinical parameters as a prognostic indicator in breast carcinoma
| Autor: | Rzymowska J |
|---|---|
| Rok vydání: | 1998 |
| Předmět: |
Adult
Cancer Research Pathology medicine.medical_specialty Silver Staining Lobular carcinoma Breast Neoplasms Biology 030218 nuclear medicine & medical imaging Flow cytometry 03 medical and health sciences 0302 clinical medicine Breast cancer Carcinoma medicine Nucleolus Organizer Region Humans Aged Neoplasm Staging Aged 80 and over Ploidies medicine.diagnostic_test Carcinoma Ductal Breast General Medicine DNA Neoplasm Middle Aged medicine.disease Flow Cytometry Prognosis Carcinoma Lobular Tissue sections Oncology Fibroadenoma 030220 oncology & carcinogenesis Lymphatic Metastasis Aneuploid Cells Female Nucleolus organizer region Breast carcinoma |
| Zdroj: | Tumori. 83(6) |
| ISSN: | 0300-8916 |
| Popis: | Silver binding nucleolar organizer regions (AgNORs) were counted in tissue sections and were shown to assist in the distinction between benign and malignant breast lesions. The mean AgNOR counts in fibroadenomas were 1.05 ± 0.85 (n=18), in lobular carcinoma 3.55 ± 0.56 (n=35), and in intraductal carcinoma 4.83 ± 1.20 (n=45). AgNOR counting may provide information on breast cancer prognosis supplementary to that obtained with other methods such as flow cytometric analysis. The AgNOR values in carcinomas were also compared with ploidy and S-phase fractions (S) by flow cytometry. More than 75% of the total cancers with counts above 3 dots per nuclear profile contained aneuploid cells, whereas 20% with counts below 3 contained diploid cells (P < 0.05). Similar trends were noticed with regard to S-phase fractions, which were 45% ± 12.5 and 14% ± 4.5, respectively, for the two groups (P < 0.05). AgNOR counting, flow cytometry and clinical parameters may provide complementary information on breast cancer prognosis. |
| Databáze: | OpenAIRE |
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