Arabidopsis MORC proteins function in the efficient establishment of RNA directed DNA methylation
| Autor: | Yasaman Jami-Alahmadi, Jihui Sha, Colette L. Picard, Ivy Kwok, Javier Gallego-Bartolomé, C. Jake Harris, Shan Hua, Zhenhui Zhong, Suhua Feng, James A. Wohlschlegel, Jason Gardiner, Steven E. Jacobsen, Ming Wang, Xueshi Cao, Yan Xue |
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| Přispěvatelé: | Zhong, Zhenhui [0000-0002-8438-0375], Harris, C Jake [0000-0001-5120-0377], Picard, Colette [0000-0002-2177-2216], Kwok, Ivy [0000-0001-6793-5618], Sha, Jihui [0000-0002-7358-1606], Jacobsen, Steven E [0000-0001-9483-138X], Apollo - University of Cambridge Repository |
| Rok vydání: | 2021 |
| Předmět: |
0106 biological sciences
Plant molecular biology Heterochromatin Science 1.1 Normal biological development and functioning Arabidopsis General Physics and Astronomy 01 natural sciences Article General Biochemistry Genetics and Molecular Biology 03 medical and health sciences chemistry.chemical_compound Underpinning research Gene Expression Regulation Plant Genetics Gene Silencing RNA-Directed DNA Methylation 030304 developmental biology Regulation of gene expression Adenosine Triphosphatases 0303 health sciences Multidisciplinary biology Arabidopsis Proteins Plant General Chemistry Methylation DNA Methylation biology.organism_classification Chromatin Cell biology Gene Expression Regulation chemistry DNA methylation Generic health relevance DNA 010606 plant biology & botany |
| Zdroj: | Nature communications, vol 12, iss 1 Nature Communications, Vol 12, Iss 1, Pp 1-13 (2021) Nature Communications |
| Popis: | The Microrchidia (MORC) family of ATPases are required for transposable element (TE) silencing and heterochromatin condensation in plants and animals, and C. elegans MORC-1 has been shown to topologically entrap and condense DNA. In Arabidopsis thaliana, mutation of MORCs has been shown to reactivate silent methylated genes and transposons and to decondense heterochromatic chromocenters, despite only minor changes in the maintenance of DNA methylation. Here we provide the first evidence localizing Arabidopsis MORC proteins to specific regions of chromatin and find that MORC4 and MORC7 are closely co-localized with sites of RNA-directed DNA methylation (RdDM). We further show that MORC7, when tethered to DNA by an artificial zinc finger, can facilitate the establishment of RdDM. Finally, we show that MORCs are required for the efficient RdDM mediated establishment of DNA methylation and silencing of a newly integrated FWA transgene, even though morc mutations have no effect on the maintenance of preexisting methylation at the endogenous FWA gene. We propose that MORCs function as a molecular tether in RdDM complexes to reinforce RdDM activity for methylation establishment. These findings have implications for MORC protein function in a variety of other eukaryotic organisms. MORC ATPases are required for transposable element silencing and heterochromatin condensation in plants and animals. Here the authors show that Arabidopsis MORCs colocalize with sites of RNA-directed DNA methylation and provide evidence that they act as molecular tethers to efficiently establish DNA methylation. |
| Databáze: | OpenAIRE |
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