Diffusivity of 125I-labelled macromolecules through collagen: mechanism of diffusion and effect of adsorption
| Autor: | Frederick H. Silver, Kevin Weadock, Donald J. Wolff |
|---|---|
| Rok vydání: | 1987 |
| Předmět: |
Diffusion
Biophysics Biocompatible Materials Bioengineering In Vitro Techniques Permeability Iodine Radioisotopes Biomaterials Adsorption Albumins Fructose-Bisphosphate Aldolase Polymer chemistry Electrochemistry medicine Animals Molecule Chemistry Hydrogen bond Angiotensin II Hydrogen Bonding Kinetics Membrane Mechanics of Materials Ceramics and Composites Thermodynamics Collagen Swelling medicine.symptom Macromolecule |
| Zdroj: | Biomaterials. 8:105-112 |
| ISSN: | 0142-9612 |
| DOI: | 10.1016/0142-9612(87)90098-6 |
| Popis: | Diffusion of angiotensin II, albumin and aldolase was studied through collagen membranes with swelling ratios between 4 and 15. The diffusion coefficient was measured from the time-lag for the onset of steadystate flux through the membrane. Binding of macromolecules to collagen was evaluated from the results of sorption studies conducted as a function of macromolecular concentration. Results presented indicate that the diffusion of macromolecules through collagen membranes is slowed by electrostatic and hydrogen bonding between individual macromolecular chains and collagen. The extent of adsorption is increased as the molecular weight of the diffusant increases. Diffusion of water soluble macromolecules through collagen occurs rapidly, suggesting that diffusion occurs through water filled channels as opposed to between collagen molecules. The results of these studies are useful in understanding diffusion through connective tissues and in the design of drug delivery systems based on collagen. |
| Databáze: | OpenAIRE |
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