Effect of macrophage blockade on the resistance of inbred mice to Paracoccidioides brasiliensis infection
| Autor: | Eva Burger, L M Singer-Vermes, Suely Sanae Kashino, Raquel dos Anjos Fazioli, Vera Lúcia Garcia Calich, Marcello Franco, Maura Moscardi-Bacchi |
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| Rok vydání: | 1995 |
| Předmět: |
Cellular immunity
Ratón Veterinary (miscellaneous) Mice Inbred Strains Applied Microbiology and Biotechnology Microbiology Lethal Dose 50 Mice Immune system Cell Movement medicine Macrophage Animals Hypersensitivity Delayed Colloids Antibodies Fungal Paracoccidioides brasiliensis biology Paracoccidioidomycosis Lethal dose Paracoccidioides Macrophage Activation biology.organism_classification medicine.disease Carbon Immunity Innate Blockade Immunoglobulin G Immunology Macrophages Peritoneal Female Disease Susceptibility Agronomy and Crop Science |
| Zdroj: | Mycopathologia. 130(3) |
| ISSN: | 0301-486X |
| Popis: | The effect of macrophage blockade on the natural resistance and on the adaptative immune response of susceptible (B10.D2/oSn) and resistant (A/Sn) mice to Paracoccidioides brasiliensis infection was investigated. B10.D2/oSn and A/Sn mice previously injected with colloidal carbon were infected ip with yeast cells to determine the 50% lethal dose, and to evaluate the anatomy and histopathology, macrophage activation, antibody production and DTH reactions. Macrophage blockade rendered both resistant and susceptible mice considerably more susceptible to infection, as evidenced by increased mortality and many disseminated lesions. P. brasiliensis infection and/or carbon treatment increased the ability of macrophages from resistant mice to spread up to 25 days after treatment. In susceptible mice the enhanced spreading capacity induced by carbon treatment was impaired at all assayed periods except at 1 week after infection. Macrophage blockade enhanced DTH reactions in resistant mice, but did not alter these reactions in susceptible mice, which remained anergic. To the contrary, macrophage blockade enhanced specific antibody production by susceptible mice, but did not affect the low levels produced by resistant mice. The effect of macrophage blockade confirms the natural tendency of resistant animals to mount DTH reactions in the course of the disease and the preferential antibody response developed by susceptible mice after P. brasiliensis infection. On the whole, macrophage functions appear to play a fundamental role in the natural and acquired resistance mechanisms to P. brasiliensis infection. |
| Databáze: | OpenAIRE |
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