Ferroportin mediates the intestinal absorption of iron from a nanoparticulate ferritin core mimetic in mice
| Autor: | Sarah J. Wilkins, Dora I. A. Pereira, Nuno Faria, Jonathan J. Powell, Greg J. Anderson, David M. Frazer, Mohamad F. Aslam, Sylvaine F. A. Bruggraber, Cornel S. G. Mirciov |
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| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
medicine.medical_specialty
Duodenum medicine.medical_treatment Ferroportin Administration Oral Biological Availability Iron supplement Biochemistry Ferric Compounds Intestinal absorption Research Communications Hemoglobins Mice Hepcidins Hepcidin Internal medicine Genetics medicine Animals Homeostasis Ferrous Compounds Molecular Biology Cation Transport Proteins Mice Knockout biology Anemia Iron-Deficiency Iron Deficiencies Bioavailability Ferritin Endocrinology medicine.anatomical_structure Enterocytes Gene Expression Regulation Intestinal Absorption nanoiron biology.protein Nanoparticles iron homeostasis Hemoglobin hepcidin basolateral export knockout mice Iron Dietary Spleen Biotechnology |
| Zdroj: | The FASEB Journal |
| ISSN: | 1530-6860 0892-6638 |
| Popis: | The ferritin core is composed of fine nanoparticulate Fe3+ oxohydroxide, and we have developed a synthetic mimetic, nanoparticulate Fe3+ polyoxohydroxide (nanoFe3+). The aim of this study was to determine how dietary iron derived in this fashion is absorbed in the duodenum. Following a 4 wk run-in on an Fe-deficient diet, mice with intestinal-specific disruption of the Fpn-1 gene (Fpn-KO), or littermate wild-type (WT) controls, were supplemented with Fe2+ sulfate (FeSO4), nanoFe3+, or no added Fe for a further 4 wk. A control group was Fe sufficient throughout. Direct intestinal absorption of nanoFe3+ was investigated using isolated duodenal loops. Our data show that FeSO4 and nanoFe3+ are equally bioavailable in WT mice, and at wk 8 the mean ± sem hemoglobin increase was 18 ± 7 g/L in the FeSO4 group and 30 ± 5 g/L in the nanoFe3+ group. Oral iron failed to be utilized by Fpn-KO mice and was retained in enterocytes, irrespective of the iron source. In summary, although nanoFe3+ is taken up directly by the duodenum its homeostasis is under the normal regulatory control of dietary iron absorption, namely via ferroportin-dependent efflux from enterocytes, and thus offers potential as a novel oral iron supplement.—Aslam, M. F., Frazer, D. M., Faria, N., Bruggraber, S. F. A., Wilkins, S. J., Mirciov, C., Powell, J. J., Anderson, G. J., Pereira, D. I. A. Ferroportin mediates the intestinal absorption of iron from a nanoparticulate ferritin core mimetic in mice. |
| Databáze: | OpenAIRE |
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