Identification of cis-acting packaging signals in the coding regions of the influenza B virus HA gene segment
| Autor: | Eliza Prangley, Louisa E. Wallace, Sophie DeFries, Karolina Punovuori, David A. Jackson, Lee Sherry |
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| Přispěvatelé: | University of St Andrews. School of Biology |
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Silent mutation RNA Untranslated viruses DNA Mutational Analysis Hemagglutinin Glycoproteins Influenza Virus QH426 Genetics Biology Genome Virus Cis acting Genome packaging Open Reading Frames 03 medical and health sciences Virology Humans Coding region Gene Packaging signals QH426 Genetics QR355 Virus Assembly biochemical phenomena metabolism and nutrition Open reading frame Influenza B virus 030104 developmental biology RNA Viral QR355 Virology |
| Popis: | For influenza A and B viruses to be infectious, they require eight viral RNA (vRNA) genome segments to be packaged into virions. For efficient packaging, influenza A viruses utilize cis-acting vRNA sequences, containing both non-coding and protein coding regions of each segment. Whether influenza B viruses have similar packaging signals is unknown. Here we show that coding regions at the 3' and 5' ends of the influenza B virus vRNA segment 4 are required for genome packaging, with the first 30 nt at each end essential for this process. Synonymous mutation of these regions led to virus attenuation, an increase in defective particle production and a reduction in packaging of multiple vRNAs. Overall, our data suggest that the influenza B virus vRNA gene segments likely interact with each other during the packaging process, which is driven by cis-acting packaging signals that extend into protein coding regions of the vRNA. Postprint |
| Databáze: | OpenAIRE |
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